Anti-p53R2 antibody - C-terminal (ab191055)
Key features and details
- Rabbit polyclonal to p53R2 - C-terminal
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p53R2 antibody - C-terminal
See all p53R2 primary antibodies -
Description
Rabbit polyclonal to p53R2 - C-terminal -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Saccharomyces cerevisiae, Caenorhabditis elegans, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Human p53R2 aa 318-332 (C terminal).
Sequence:EGKTNFFEKRVSEYQ
Database link: Q7LG56 -
Positive control
- Rat thymus tissue lysates: MCF7, A431 and HeLa cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab191055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 41 kDa. |
Target
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Function
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. -
Tissue specificity
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. -
Pathway
Genetic information processing; DNA replication. -
Involvement in disease
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase small chain family. -
Cellular localization
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 50484 Human
- Entrez Gene: 382985 Mouse
- Entrez Gene: 299976 Rat
- Entrez Gene: 853427 Saccharomyces cerevisiae
- Omim: 604712 Human
- SwissProt: P42170 Caenorhabditis elegans
- SwissProt: Q4R741 Cynomolgus monkey
- SwissProt: Q7LG56 Human
see all -
Alternative names
- DKFZp686M05248 antibody
- MGC102856 antibody
- MGC42116 antibody
see all
Images
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Western blot - Anti-p53R2 antibody - C-terminal (ab191055)All lanes : Anti-p53R2 antibody - C-terminal (ab191055) at 0.5 µg/ml
Lane 1 : Rat thymus tissue lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 41 kDa
References (0)
ab191055 has not yet been referenced specifically in any publications.
