1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
1/10 - 1/100.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
Genetic information processing; DNA replication.
Involvement in disease
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy. Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Belongs to the ribonucleoside diphosphate reductase small chain family.
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
p53 R2 antibody
p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
Ribonucleoside diphosphate reductase M2 subunit B antibody
Ribonucleoside-diphosphate reductase subunit M2 B antibody
Ribonucleotide reductase M2 B (TP53 inducible) antibody
Ribonucleotide reductase M2 B antibody
Ribonucleotide reductase small subunit like 2 p53 inducible antibody
RRM 2B antibody
TP53 inducible ribonucleotide reductase M2 B antibody
TP53-inducible ribonucleotide reductase M2 B antibody
Western blot - Anti-p53R2 antibody [EPR8816] (ab154194)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: p53R2 knockout HAP1 cell lysate (20 µg) Lane 3: MCF7 cell lysate (20 µg) Lane 4: SW480 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab154194 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa. ab154194 was shown to recognize p53R2 when p53R2 knockout samples were used, along with additional cross-reactive bands. Wild-type and p53R2 knockout samples were subjected to SDS-PAGE. ab154194 and ab18058 (loading control to Vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.