Overview

  • Product name

    Anti-p53R2 antibody [EPR8816] (HRP)
    See all p53R2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8816] to p53R2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human p53R2 (N terminal).
    Database link: Q7LG56

  • Positive control

    • WB: SW480 and wildtype HAP1 whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
  • Tissue specificity

    Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
  • Pathway

    Genetic information processing; DNA replication.
  • Involvement in disease

    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
    Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686M05248 antibody
    • MGC102856 antibody
    • MGC42116 antibody
    • MTDPS8A antibody
    • MTDPS8B antibody
    • p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
    • p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
    • P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
    • p53 R2 antibody
    • p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
    • p53R2 antibody
    • Ribonucleoside diphosphate reductase M2 subunit B antibody
    • Ribonucleoside-diphosphate reductase subunit M2 B antibody
    • Ribonucleotide reductase M2 B (TP53 inducible) antibody
    • Ribonucleotide reductase M2 B antibody
    • Ribonucleotide reductase small subunit like 2 p53 inducible antibody
    • RIR2B_HUMAN antibody
    • RRM 2B antibody
    • RRM2B antibody
    • TP53 inducible ribonucleotide reductase M2 B antibody
    • TP53-inducible ribonucleotide reductase M2 B antibody
    see all

Images

  • All lanes : Anti-p53R2 antibody [EPR8816] (HRP) (ab206095) at 1/1000 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Wild-type HAP1 cell lysate at 20 µg
    Lane 3 : p53R2 knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206095 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab206095 has not yet been referenced specifically in any publications.

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