Anti-p57 Kip2 antibody [EP2516] (ab119989)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-p57 Kip2 antibody [EP2516]
See all p57 Kip2 primary antibodies -
Description
Rabbit monoclonal [EP2516] to p57 Kip2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human p57 Kip2 aa 250-350 (internal sequence). The exact sequence is proprietary.
-
Positive control
- HeLa cell lysate, treated with Dexamethasone.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2516 -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab119989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa). |
Target
-
Function
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. -
Tissue specificity
Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. -
Involvement in disease
Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
Note=Defects in CDKN1C are involved in tumor formation. -
Sequence similarities
Belongs to the CDI family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1028 Human
- Omim: 600856 Human
- SwissProt: P49918 Human
- Unigene: 106070 Human
-
Alternative names
- Beckwith Wiedemann syndrome antibody
- BWCR antibody
- BWS antibody
see all
Images
-
![Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)](https://www.abcam.com/ps/products/119/ab119989/Images/ab119989-133587-anti-p57-kip2-antibody-ep2516-western-blot.jpg)
Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Dexamethasone
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Protocols
Datasheets and documents
References
ab119989 has not yet been referenced specifically in any publications.
