Anti-p57 Kip2 antibody [EP2516] (ab119989)
![Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)](https://www.abcam.com/ps/products/119/ab119989/Images/ab119989-478853-anti-p57-kip2-antibody-ep2516-western-blot-wildtype-hela-vehicle-control-dexamethasone-0-nm-16-h-ab277359-wildty.jpg "Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)")
Key features and details
- Rabbit monoclonal [EP2516] to p57 Kip2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-p57 Kip2 antibody [EP2516]
See all p57 Kip2 primary antibodies -
Description
Rabbit monoclonal [EP2516] to p57 Kip2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate, treated with Dexamethasone, Wild-type HeLa Treated Dexamethasone
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2516 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. -
Tissue specificity
Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. -
Involvement in disease
Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
Note=Defects in CDKN1C are involved in tumor formation. -
Sequence similarities
Belongs to the CDI family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1028 Human
- Omim: 600856 Human
- SwissProt: P49918 Human
- Unigene: 106070 Human
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Alternative names
- Beckwith Wiedemann syndrome antibody
- BWCR antibody
- BWS antibody
see all
Images
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Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution
Lane 1 : Wild-type HeLa Vehicle Control Dexamethasone (0 nM, 16 h) ab277359 cell lysate
Lane 2 : Wild-type HeLa Treated Dexamethasone (50 nM, 16 h) ab287335 cell lysate
Lane 3 : CDKN1C knockout HeLa Vehicle Control Dexamethasone (0 nM, 16 h) ab277299 cell lysate
Lane 4 : CDKN1C knockout HeLa Treated Dexamethasone (50 nM, 16 h) ab281877 cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-p57 Kip2 antibody [EP2516] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab119989 was shown to bind specifically to p57 Kip2. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in CDKN1C knockout cell line ab280061 (knockout cell lysate ab280120). To generate this image, wild-type and CDKN1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.
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![Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)](https://www.abcam.com/ps/products/119/ab119989/Images/ab119989-133587-anti-p57-kip2-antibody-ep2516-western-blot.jpg)
Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Dexamethasone
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Datasheets and documents
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Datasheet download
References (1)
ab119989 has been referenced in 1 publication.
- Varricchio L et al. TGF-ß1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosis. JCI Insight 6:N/A (2021). PubMed: 34383713
