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  1. Link

    p57-kip2-antibody-ep2516-ab119989.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Cip / Kip
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Validated using a knockout cell lineRabMAb

Anti-p57 Kip2 antibody [EP2516] (ab119989)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)
  • Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)

Key features and details

  • Rabbit monoclonal [EP2516] to p57 Kip2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-p57 Kip2 antibody [EP2516]
    See all p57 Kip2 primary antibodies
  • Description

    Rabbit monoclonal [EP2516] to p57 Kip2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate, treated with Dexamethasone, Wild-type HeLa Treated Dexamethasone
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2516
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Cip/kip

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CDKN1C (p57 Kip2) knockout HeLa cell line (ab280061)
  • KO cell lysates

    • Human CDKN1C (p57 Kip2) knockout HeLa cell lysate (ab280120)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
  • Tissue specificity

    Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
  • Involvement in disease

    Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
    Note=Defects in CDKN1C are involved in tumor formation.
  • Sequence similarities

    Belongs to the CDI family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P49918 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1028 Human
    • Omim: 600856 Human
    • SwissProt: P49918 Human
    • Unigene: 106070 Human
    • Alternative names

      • Beckwith Wiedemann syndrome antibody
      • BWCR antibody
      • BWS antibody
      • CDKI antibody
      • CDKN 1C antibody
      • CDKN1C antibody
      • CDN1C_HUMAN antibody
      • Cyclin dependent kinase inhibitor 1C antibody
      • Cyclin dependent kinase inhibitor p57 antibody
      • Cyclin-dependent kinase inhibitor 1C antibody
      • Cyclin-dependent kinase inhibitor p57 antibody
      • KIP 2 antibody
      • KIP2 antibody
      • p57 antibody
      • p57 Kip 2 antibody
      • p57KIP2 antibody
      • WBS antibody
      see all

    Images

    • Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)
      Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)
      All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution

      Lane 1 : Wild-type HeLa Vehicle Control Dexamethasone (0 nM, 16 h) ab277359 cell lysate
      Lane 2 : Wild-type HeLa Treated Dexamethasone (50 nM, 16 h) ab287335 cell lysate
      Lane 3 : CDKN1C knockout HeLa Vehicle Control Dexamethasone (0 nM, 16 h) ab277299 cell lysate
      Lane 4 : CDKN1C knockout HeLa Treated Dexamethasone (50 nM, 16 h) ab281877 cell lysate
      Lane 5 : MCF7 cell lysate
      Lane 6 : SH-SY5Y cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 32 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-p57 Kip2 antibody [EP2516] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab119989 was shown to bind specifically to p57 Kip2. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in CDKN1C knockout cell line ab280061 (knockout cell lysate ab280120). To generate this image, wild-type and CDKN1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

    • Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)
      Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)
      All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Dexamethasone

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 32 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab119989? Please let us know so that we can cite the reference in this datasheet.

    ab119989 has been referenced in 1 publication.

    • Varricchio L  et al. TGF-ß1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosis. JCI Insight 6:N/A (2021). PubMed: 34383713

    Customer reviews and Q&As

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