- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-p57 Kip2 antibody [EP2516]
See all p57 Kip2 primary antibodies -
DescriptionRabbit monoclonal [EP2516] to p57 Kip2
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human p57 Kip2 aa 250-350 (internal sequence). The exact sequence is proprietary.
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Positive control
- HeLa cell lysate, treated with Dexamethasone.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP2516
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab119989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 32 kDa). |
Target
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FunctionPotent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
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Tissue specificityExpressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
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Involvement in diseaseDefects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
Note=Defects in CDKN1C are involved in tumor formation. -
Sequence similaritiesBelongs to the CDI family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1028 Human
- Omim: 600856 Human
- SwissProt: P49918 Human
- Unigene: 106070 Human
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Alternative names
- Beckwith Wiedemann syndrome antibody
- BWCR antibody
- BWS antibody
see all
Images
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![Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)](https://www.abcam.com/ps/datasheet/images/119/ab119989/p57-Kip2-Primary-antibodies-ab119989-1.jpg)
Western blot - Anti-p57 Kip2 antibody [EP2516] (ab119989)All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Dexamethasone
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Protocols
References
ab119989 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"