Overview

  • Product name

    Anti-p57 Kip2 antibody [EP2718(2)]
    See all p57 Kip2 primary antibodies
  • Description

    Rabbit monoclonal [EP2718(2)] to p57 Kip2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human p57 Kip2 protein (P49918).

  • Positive control

    • HeLa treated with dexamethasone, HeLa, SH-SY5Y, and 293T cell lysates; Human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
    • Tissue specificity

      Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
    • Involvement in disease

      Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
      Note=Defects in CDKN1C are involved in tumor formation.
    • Sequence similarities

      Belongs to the CDI family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beckwith Wiedemann syndrome antibody
      • BWCR antibody
      • BWS antibody
      • CDKI antibody
      • CDKN 1C antibody
      • CDKN1C antibody
      • CDN1C_HUMAN antibody
      • Cyclin dependent kinase inhibitor 1C antibody
      • Cyclin dependent kinase inhibitor p57 antibody
      • Cyclin-dependent kinase inhibitor 1C antibody
      • Cyclin-dependent kinase inhibitor p57 antibody
      • KIP 2 antibody
      • KIP2 antibody
      • p57 antibody
      • p57 Kip 2 antibody
      • p57KIP2 antibody
      • WBS antibody
      see all

    Images

    • All lanes : Anti-p57 Kip2 antibody [EP2718(2)] (ab133531) at 1/1000 dilution

      Lane 1 : Lysate of HeLa cells treated with dexamethasone
      Lane 2 : HeLa cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemistry analysis of Paraffin Embedded Human placenta tissue labelling p57 Kip2 with ab133531 at 1/100.

    References

    This product has been referenced in:

    • Bazot Q  et al. Epstein-Barr Virus Proteins EBNA3A and EBNA3C Together Induce Expression of the Oncogenic MicroRNA Cluster miR-221/miR-222 and Ablate Expression of Its Target p57KIP2. PLoS Pathog 11:e1005031 (2015). Read more (PubMed: 26153983) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 02 2018

    Application
    Western blot
    Sample
    Human Cell lysate - other (Lymphoblastoid cell line - EBNA3A KO)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    Lymphoblastoid cell line - EBNA3A KO
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Quentin Bazot

    Verified customer

    Submitted Aug 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up