Overview

  • Product name

  • Description

    Rabbit polyclonal to P5CS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human P5CS aa 575-625. The exact sequence is proprietary. from Isoform Short
    Sequence:

    MGHSEG ICHMYVDSEA SVDKVTRLVR DSKCEYPAAC NALETLLIHR DLLRT


    Database link: P54886-2

  • Positive control

    • Whole cell lysate from HeLa, 293T, Jurkat, TCMK and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab206682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Pathway

    Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.
    Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
  • Involvement in disease

    Defects in ALDH18A1 are the cause of mental retardation-joint hypermobility-skin laxity with or without metabolic abnormalities (MRJHSL) [MIM:612652]. Clinical manifestations include microcephaly, progressive neurologic dysfunction, mental retardation, progeroid appearance, joint hypermobility, skin laxity and hyperelasticity, cataracts. Some patients manifest metabolic disturbances such as hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia.
  • Sequence similarities

    In the N-terminal section; belongs to the glutamate 5-kinase family.
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Form

    P5CS catalyzes the ATP- and NADPH-dependent conversion of L-glutamate to glutamic gamma-semialdehyde, which is the metabolic precursor for proline biosynthesis. There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • 2810433K04Rik antibody
    • AI429789 antibody
    • Aldehyde dehydrogenase 18 family member A1 antibody
    • Aldehyde dehydrogenase 18A1 antibody
    • Aldehyde dehydrogenase family 18 member A1 antibody
    • ALDH18A1 antibody
    • Delta 1 pyrroline 5 carboxylate synthetase antibody
    • Delta1 pyrroline 5 carboxlate synthetase antibody
    • Gamma-glutamyl kinase antibody
    • Gamma-glutamyl phosphate reductase antibody
    • GK antibody
    • Glutamate-5-semialdehyde dehydrogenase antibody
    • Glutamyl-gamma-semialdehyde dehydrogenase antibody
    • GPR antibody
    • GSAS antibody
    • MGC117316 antibody
    • MGC32233 antibody
    • P5CS antibody
    • P5CS_HUMAN antibody
    • PYCS antibody
    • Pyrroline 5 carboxylate synthetase (glutamate gamma semialdehyde synthetase) antibody
    see all

Images

  • All lanes : Anti-P5CS antibody (ab206682) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 10 seconds


    Samples prepared using NETN lysis buffer.

  • Detection of Human P5CS by Western Blot of Immunoprecipitates.

    Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.

    Lane 1: ab206682 used for IP at 6 μg per reaction.  For blotting immunoprecipitated P5CS, ab206682 was used at 0.4 μg/mL.

    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab206682 has not yet been referenced specifically in any publications.

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