Anti-P5CS antibody (ab223713)
Key features and details
- Rabbit polyclonal to P5CS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-P5CS antibody
See all P5CS primary antibodies -
Description
Rabbit polyclonal to P5CS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human P5CS aa 50-250.
Database link: P54886 -
Positive control
- WB: NIH/3T3, NBT-II and U-251 cell lysates. IHC-P: Human colon, testis and cerebral cortex tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 87 kDa.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 87 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Pathway
Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.
Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. -
Involvement in disease
Defects in ALDH18A1 are the cause of mental retardation-joint hypermobility-skin laxity with or without metabolic abnormalities (MRJHSL) [MIM:612652]. Clinical manifestations include microcephaly, progressive neurologic dysfunction, mental retardation, progeroid appearance, joint hypermobility, skin laxity and hyperelasticity, cataracts. Some patients manifest metabolic disturbances such as hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia. -
Sequence similarities
In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 5832 Human
- Entrez Gene: 56454 Mouse
- Entrez Gene: 100173488 Orangutan
- Entrez Gene: 361755 Rat
- Omim: 138250 Human
- SwissProt: P54886 Human
- SwissProt: Q9Z110 Mouse
- SwissProt: Q5R4M8 Orangutan
see all -
Form
P5CS catalyzes the ATP- and NADPH-dependent conversion of L-glutamate to glutamic gamma-semialdehyde, which is the metabolic precursor for proline biosynthesis. There are 2 isoforms produced by alternative splicing. -
Alternative names
- 2810433K04Rik antibody
- AI429789 antibody
- Aldehyde dehydrogenase 18 family member A1 antibody
see all
Images
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Paraffin embedded sections of human testis were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human cerebral cortex were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 87 kDa -
All lanes : Anti-P5CS antibody (ab223713) at 1/20 dilution
Lane 1 : Caco-2 cell lysate transfected with control siRNA
Lane 2 : Caco-2 cell lysate transfected with target specific siRNA probe #1
Lane 3 : Caco-2 cell lysate transfected with target specific siRNA probe #2
Predicted band size: 87 kDa -
All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat bladder tumor cell line) cell lysate
Predicted band size: 87 kDa -
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223713 has not yet been referenced specifically in any publications.