Key features and details
- Rabbit polyclonal to p60 CAF1/MPP7
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p60 CAF1/MPP7 antibody
See all p60 CAF1/MPP7 primary antibodies
DescriptionRabbit polyclonal to p60 CAF1/MPP7
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human p60 CAF1/MPP7.
Database link: NP_005432.1
- Whole cell lysate from Hela cells.
This product was previously labelled as p60 CAF1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
Sequence similaritiesBelongs to the WD repeat HIR1 family.
Contains 7 WD repeats.
Developmental stageActive complex is found in G1, S and G2 phases.
modificationsDifferentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
- Information by UniProt
- CAF 1 subunit B antibody
- CAF 1A antibody
- CAF I 60 kDa subunit antibody
ab72519 has not yet been referenced specifically in any publications.