Product nameAnti-p60 CAF1/MPP7 antibody
See all p60 CAF1/MPP7 primary antibodies
DescriptionRabbit polyclonal to p60 CAF1/MPP7
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human p60 CAF1/MPP7 (C terminal).
Database link: NP_005432.1
- Whole cell lysate from Hela and 293T cells.
This product was previously labelled as p60 CAF1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
Sequence similaritiesBelongs to the WD repeat HIR1 family.
Contains 7 WD repeats.
Developmental stageActive complex is found in G1, S and G2 phases.
modificationsDifferentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
- Information by UniProt
- CAF 1 subunit B antibody
- CAF 1A antibody
- CAF I 60 kDa subunit antibody
All lanes : Anti-p60 CAF1/MPP7 antibody (ab72520) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 240 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1/MPP7 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
ab72520 staining p60 CAF1/MPP7 in human PC3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunoprecipitation/ Western Blot of p60 CAF1/MPP7. Lane 1: ab72520 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72520 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
ab72520 has been referenced in 1 publication.
- Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). PubMed: 27794043