Recombinant
RabMAb

Recombinant Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

Overview

  • Product name

    Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free
    See all p60 CAF1/MPP7 primary antibodies
  • Description

    Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide within Human p60 CAF1/MPP7 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13112

  • Positive control

    • Jurkat, HeLa, 293T, and Caco2 cell lysates; Human colon and tonsil tissues.
  • General notes

    ab226065 is the carrier-free version of ab109442 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab226065 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as p60 CAF1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6105
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
  • Sequence similarities

    Belongs to the WD repeat HIR1 family.
    Contains 7 WD repeats.
  • Developmental stage

    Active complex is found in G1, S and G2 phases.
  • Post-translational
    modifications

    Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • Cellular localization

    Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAF 1 subunit B antibody
    • CAF 1A antibody
    • CAF I 60 kDa subunit antibody
    • CAF IP60 antibody
    • CAF-1 antibody
    • CAF-1 subunit B antibody
    • CAF-I 60 kDa subunit antibody
    • CAF-I p60 antibody
    • CAF1 antibody
    • CAF1A antibody
    • CAF1B_HUMAN antibody
    • CAF1P60 antibody
    • CHAF 1B antibody
    • CHAF1B antibody
    • Chromatin assembly factor 1 subunit B antibody
    • Chromatin assembly factor I antibody
    • Chromatin assembly factor I p60 subunit antibody
    • Human chromatin assembly factor-I p60 subunit antibody
    • M phase phosphoprotein 7 antibody
    • M-phase phosphoprotein 7 antibody
    • MPHOSPH7 antibody
    • MPP 7 antibody
    • MPP7 antibody
    • p60 subunit antibody
    see all

References

ab226065 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226065.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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