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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Anti-p63 antibody [C24-I] - N-terminal (ab179874)

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Western blot - Anti-p63 antibody [C24-I] - N-terminal (ab179874)

    Key features and details

    • Rabbit monoclonal [C24-I] to p63 - N-terminal
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-p63 antibody [C24-I] - N-terminal
      See all p63 primary antibodies
    • Description

      Rabbit monoclonal [C24-I] to p63 - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human p63 (N terminal). The exact sequence is proprietary.
      Database link: Q9H3D4

    • Epitope

      Antibody recognizes the epitope located between Val69 - Lys88
    • Positive control

      • Recombinant tagged Human AMACR + p63 (aa 1 – 680).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 8.00
      Preservative: 0.05% Sodium azide
      Constituents: 0.32% Tris HCl, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      C24-I
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • p53
      • Cell Biology
      • Apoptosis
      • Intracellular
      • p53 Pathway
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • p53
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • p53
      • Cancer
      • Cell cycle
      • Cell cycle inhibitors
      • p53 pathway
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • p53 pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab179874 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000. Predicted molecular weight: 77 kDa.
    Notes
    WB
    1/1000. Predicted molecular weight: 77 kDa.

    Target

    • Function

      Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
    • Tissue specificity

      Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
    • Involvement in disease

      Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
      Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
      Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
      Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
      Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
      Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
      Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
      Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
    • Sequence similarities

      Belongs to the p53 family.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain

      The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
    • Post-translational
      modifications

      May be sumoylated.
      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9H3D4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8626 Human
      • Omim: 603273 Human
      • SwissProt: Q9H3D4 Human
      • Unigene: 137569 Human
      • Alternative names

        • AIS antibody
        • Amplified in squamous cell carcinoma antibody
        • B(p51A) antibody
        • B(p51B) antibody
        • Chronic ulcerative stomatitis protein antibody
        • CUSP antibody
        • DN p63 alpha 1 antibody
        • DNp63 antibody
        • EEC3 antibody
        • id:ibd3516 antibody
        • Keratinocyte transcription factor antibody
        • Keratinocyte transcription factor KET antibody
        • KET antibody
        • LMS antibody
        • MGC115972 antibody
        • MGC192897 antibody
        • NBP antibody
        • OFC8 antibody
        • OTTHUMP00000209732 antibody
        • OTTHUMP00000209733 antibody
        • OTTHUMP00000209734 antibody
        • OTTHUMP00000209735 antibody
        • OTTHUMP00000209737 antibody
        • OTTHUMP00000209738 antibody
        • OTTHUMP00000209739 antibody
        • OTTHUMP00000209740 antibody
        • OTTHUMP00000209741 antibody
        • OTTHUMP00000209742 antibody
        • OTTHUMP00000209743 antibody
        • OTTHUMP00000209744 antibody
        • p40 antibody
        • p51 antibody
        • P51/P63 antibody
        • p53-related protein p63 antibody
        • p53CP antibody
        • p63 antibody
        • P63_HUMAN antibody
        • p73H antibody
        • p73L antibody
        • RHS antibody
        • SHFM4 antibody
        • TAp63alpha antibody
        • TP53CP antibody
        • TP53L antibody
        • TP63 antibody
        • TP73L antibody
        • Transformation related protein 63 antibody
        • Transformation-related protein 63 antibody
        • Trp53rp1 antibody
        • Trp63 antibody
        • Tumor protein 63 antibody
        • Tumor protein p53-competing protein antibody
        • Tumor protein p53-like antibody
        • Tumor protein p63 antibody
        • Tumor protein p63 deltaN isoform delta antibody
        • Tumor protein p73 antibody
        • Tumor protein p73-like antibody
        see all

      Images

      • Western blot - Anti-p63 antibody [C24-I] - N-terminal (ab179874)
        Western blot - Anti-p63 antibody [C24-I] - N-terminal (ab179874)
        All lanes : Anti-p63 antibody [C24-I] - N-terminal (ab179874) at 1/1000 dilution

        Lane 1 : Recombinant tagged Human AMACR + p63 (aa 1 – 680) at 0.1 µg
        Lane 2 : Recombinant tagged Human AMACR + p63 (aa 1 – 680) at 0.2 µg
        Lane 3 : Recombinant tagged Human AMACR + p63 (aa 1 – 680) at 0.5 µg

        Predicted band size: 77 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab179874? Please let us know so that we can cite the reference in this datasheet.

      ab179874 has not yet been referenced specifically in any publications.

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