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Synthetic peptide within Human p63 aa 1-250. The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab124762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 50-75 kDa (predicted molecular weight: 77 kDa).
For unpurified, use 1/200.
|IHC-P||1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified, use 1/2500.
For unpurified, use 1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Unpurified ab124762 showing positive staining in prostate hyperplasia tissue.
Immunofluorescent staining of A431 cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab124762 at a dilution of 1/60. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Overlay histogram showing A431 cells stained with unpurified ab124762 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab124762, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical staining of paraffin embedded human prostate with purified ab124762 at a working dilution of 1 in 12000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Unpurified ab124762 staining p63 in Human corneal limbal epithelial cells (primary culture) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with 0.3% Triton X-100 for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS + 10% Goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunohistochemical staining of p63 in paraffin-embedded human cervical carcinoma tissue with unpurified ab124762, at a 1/500 dilution.
Immunofluorescent staining of A431 cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab124762 at a dilution of 1/60. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Unpurified ab124762 showing positive staining in normal tonsil tissue.
Unpurified ab124762 showing negative staining in skeletal muscle tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"