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    p63-antibody-epr5701-ab124762.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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RecombinantRabMAb

Recombinant Anti-p63 antibody [EPR5701] (ab124762)

  • Datasheet
  • SDS
Reviews (10)Q&A (1)References (69)

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Western blot - Anti-p63 antibody [EPR5701] (ab124762)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (ab124762)
  • Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)
  • Flow Cytometry (Intracellular) - Anti-p63 antibody [EPR5701] (ab124762)
  • Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)
  • Anti-p63 antibody [EPR5701] (ab124762)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5701] to p63
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Biotin Carrier Free HRP

You may also be interested in

Primary
Product image
Biotin Anti-p63 antibody [EPR5701] (ab202861)
ELISA
Product image
Human P63 ELISA Kit (ab234564)

View more associated products

Overview

  • Product name

    Anti-p63 antibody [EPR5701]
    See all p63 primary antibodies
  • Description

    Rabbit monoclonal [EPR5701] to p63
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human p63 aa 1-250. The exact sequence is proprietary.
    Database link: Q9H3D4

  • Positive control

    • WB: Rat bladder and skin, Mouse bladder, skin and thymus tissue lysates; HaCaT, PC-12, and A431 cell lysates; IHC-P: Human breast tissue; ICC/IF: A431 and primary corneal limbal cells; Flow Cyt (intra): A431 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5701
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Associated products

  • Alternative Versions

    • HRP Anti-p63 antibody [EPR5701] (ab202357)
    • Biotin Anti-p63 antibody [EPR5701] (ab202861)
    • Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    • Alexa Fluor® 488 Anti-p63 antibody [EPR5701] (ab246727)
    • Alexa Fluor® 647 Anti-p63 antibody [EPR5701] (ab246728)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • PC-12 nuclear extract lysate (ab14884)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/80.

For unpurified, use 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/1000. Detects a band of approximately 50-75 kDa (predicted molecular weight: 77 kDa).

For unpurified, use 1/200.

IHC-P (5)
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/2500.

See IHC antigen retrival protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF (1)
1/200.

For unpurified, use 1/60.

Notes
Flow Cyt (Intra)
1/80.

For unpurified, use 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 50-75 kDa (predicted molecular weight: 77 kDa).

For unpurified, use 1/200.

IHC-P
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/2500.

See IHC antigen retrival protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF
1/200.

For unpurified, use 1/60.

Target

  • Function

    Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
  • Tissue specificity

    Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
  • Involvement in disease

    Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
    Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
    Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
    Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
    Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
    Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
    Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
    Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similarities

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain

    The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
  • Post-translational
    modifications

    May be sumoylated.
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9H3D4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8626 Human
    • Entrez Gene: 22061 Mouse
    • Entrez Gene: 246334 Rat
    • Omim: 603273 Human
    • SwissProt: Q9H3D4 Human
    • SwissProt: O88898 Mouse
    • SwissProt: Q9JJP6 Rat
    • Unigene: 137569 Human
    • Unigene: 20894 Mouse
    • Unigene: 42907 Rat
    see all
  • Alternative names

    • AIS antibody
    • Amplified in squamous cell carcinoma antibody
    • B(p51A) antibody
    • B(p51B) antibody
    • Chronic ulcerative stomatitis protein antibody
    • CUSP antibody
    • DN p63 alpha 1 antibody
    • DNp63 antibody
    • EEC3 antibody
    • id:ibd3516 antibody
    • Keratinocyte transcription factor antibody
    • Keratinocyte transcription factor KET antibody
    • KET antibody
    • LMS antibody
    • MGC115972 antibody
    • MGC192897 antibody
    • NBP antibody
    • OFC8 antibody
    • OTTHUMP00000209732 antibody
    • OTTHUMP00000209733 antibody
    • OTTHUMP00000209734 antibody
    • OTTHUMP00000209735 antibody
    • OTTHUMP00000209737 antibody
    • OTTHUMP00000209738 antibody
    • OTTHUMP00000209739 antibody
    • OTTHUMP00000209740 antibody
    • OTTHUMP00000209741 antibody
    • OTTHUMP00000209742 antibody
    • OTTHUMP00000209743 antibody
    • OTTHUMP00000209744 antibody
    • p40 antibody
    • p51 antibody
    • P51/P63 antibody
    • p53-related protein p63 antibody
    • p53CP antibody
    • p63 antibody
    • P63_HUMAN antibody
    • p73H antibody
    • p73L antibody
    • RHS antibody
    • SHFM4 antibody
    • TAp63alpha antibody
    • TP53CP antibody
    • TP53L antibody
    • TP63 antibody
    • TP73L antibody
    • Transformation related protein 63 antibody
    • Transformation-related protein 63 antibody
    • Trp53rp1 antibody
    • Trp63 antibody
    • Tumor protein 63 antibody
    • Tumor protein p53-competing protein antibody
    • Tumor protein p53-like antibody
    • Tumor protein p63 antibody
    • Tumor protein p63 deltaN isoform delta antibody
    • Tumor protein p73 antibody
    • Tumor protein p73-like antibody
    see all

Images

  • Western blot - Anti-p63 antibody [EPR5701] (ab124762)
    Western blot - Anti-p63 antibody [EPR5701] (ab124762)
    All lanes : Anti-p63 antibody [EPR5701] (ab124762) at 1/1000 dilution (Purified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse skin lysates
    Lane 4 : Mouse thymus lysates
    Lane 5 : Mouse bladder lysates
    Lane 6 : Rat skin lysates
    Lane 7 : Rat bladder lysates
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 37-75 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

    The bands observed are consistent with what have been described in PMID 30649915 as isoforms of p63.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (ab124762)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (ab124762)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling p63 with purified ab124762 at 1/5000 dilution (0.16 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)
    Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)

    Immunocytochemistry/ Immunofluorescence analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling p63 with Purified ab124762 at 1/200 dilution (4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-p63 antibody [EPR5701] (ab124762)
    Flow Cytometry (Intracellular) - Anti-p63 antibody [EPR5701] (ab124762)

    Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling p63 with Purified ab124762 at 1/80 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)
    Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [EPR5701] (ab124762)This image is courtesy of an Abreview submitted by Manuel Chacon

    Unpurified ab124762 staining p63 in Human corneal limbal epithelial cells (primary culture) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with 0.3% Triton X-100 for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS + 10% Goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-p63 antibody [EPR5701] (ab124762)
    Anti-p63 antibody [EPR5701] (ab124762)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (69)

Publishing research using ab124762? Please let us know so that we can cite the reference in this datasheet.

ab124762 has been referenced in 69 publications.

  • Tong X  et al. Nanog maintains stemness of Lkb1-deficient lung adenocarcinoma and prevents gastric differentiation. EMBO Mol Med 13:e12627 (2021). PubMed: 33439550
  • Lena AM  et al. The p63 C-terminus is essential for murine oocyte integrity. Nat Commun 12:383 (2021). PubMed: 33452256
  • Li J  et al. SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation. Nat Commun 12:784 (2021). PubMed: 33542242
  • Widowski H  et al. Sequential Exposure to Antenatal Microbial Triggers Attenuates Alveolar Growth and Pulmonary Vascular Development and Impacts Pulmonary Epithelial Stem/Progenitor Cells. Front Med (Lausanne) 8:614239 (2021). PubMed: 33693012
  • Bae Y  et al. miR-30c-1 encourages human corneal endothelial cells to regenerate through ameliorating senescence. Aging (Albany NY) 13:9348-9372 (2021). PubMed: 33744867
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 10 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p63 antibody [EPR5701]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (skin)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
skin
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Emmanuel Bouchaert

Verified customer

Submitted Feb 28 2022

Immunohistochemistry (Frozen sections) abreview for Anti-p63 antibody [EPR5701]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (lung)
Permeabilization
Yes - 0.4% triton x-100
Specification
lung
Blocking step
eBioscience™ IHC /ICC Blocking Buffer - Low Protein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 26 2021

ChIP abreview for Anti-p63 antibody [EPR5701]

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (esophagus)
Specification
esophagus
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 55 minute(s) and 0 second(s)
Specification of the cross-linking agent: DSG (pierce) for 45 minutes, t
Positive control
my samples
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 30 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p63 antibody [EPR5701]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (ovary)
Antigen retrieval step
None
Permeabilization
No
Specification
ovary
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. qiuxia liang

Verified customer

Submitted Apr 09 2018

Western blot abreview for Anti-p63 antibody [EPR5701]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (ovary)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
ovary
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 06 2018

Western blot abreview for Anti-p63 antibody [EPR5701]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human primary airway epithelial and fibroblast cel)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Human primary airway epithelial and fibroblast cel
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Aaron Gardner

Verified customer

Submitted Oct 24 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p63 antibody [EPR5701]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Primary pulmonary epithelial cells cultured at air)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate
Permeabilization
Yes - 0.1% Triton-X 100 for 30 minutes at room temperature.
Specification
Primary pulmonary epithelial cells cultured at air
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Aaron Gardner

Verified customer

Submitted Oct 13 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-p63 [EPR5701] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Corneal Limbal epithelial cells (primary culture))
Specification
Corneal Limbal epithelial cells (primary culture)
Permeabilization
Yes - Triton 0,3% - 5 minutes
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Manuel Chacon

Verified customer

Submitted Dec 12 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p63 [EPR5701] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample
Mouse Tissue sections (ear section)
Specification
ear section
Permeabilization
Yes - 0,1% Tween, 1% BSA, 1% Horse serum
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jul 30 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p63 [EPR5701] antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (eyes)
Specification
eyes
Fixative
Davidson's
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 08 2013

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