• Product name
    Anti-p63 antibody [Y289]
    See all p63 primary antibodies
  • Description
    Rabbit monoclonal [Y289] to p63
  • Host species
  • Specificity
    This antibody should detect both isoforms 2,9,10,11 and 12 of p63.
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human p63 aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Epitope
    ab32353 reacts with an epitope located in the C terminal region of p63.
  • Positive control
    • WB: A431 cell lysate. IHC-P: Human skin tissue. ICC/IF: A431 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 77 kDa.

For unpurified use at 1/4000.

ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.

Flow Cyt Use at an assay dependent concentration.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
    • Tissue specificity
      Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
    • Involvement in disease
      Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
      Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
      Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
      Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
      Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
      Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
      Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
      Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
    • Sequence similarities
      Belongs to the p53 family.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain
      The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
    • Post-translational
      May be sumoylated.
      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • AIS antibody
      • Amplified in squamous cell carcinoma antibody
      • B(p51A) antibody
      • B(p51B) antibody
      • Chronic ulcerative stomatitis protein antibody
      • CUSP antibody
      • DN p63 alpha 1 antibody
      • DNp63 antibody
      • EEC3 antibody
      • id:ibd3516 antibody
      • Keratinocyte transcription factor antibody
      • Keratinocyte transcription factor KET antibody
      • KET antibody
      • LMS antibody
      • MGC115972 antibody
      • MGC192897 antibody
      • NBP antibody
      • OFC8 antibody
      • OTTHUMP00000209732 antibody
      • OTTHUMP00000209733 antibody
      • OTTHUMP00000209734 antibody
      • OTTHUMP00000209735 antibody
      • OTTHUMP00000209737 antibody
      • OTTHUMP00000209738 antibody
      • OTTHUMP00000209739 antibody
      • OTTHUMP00000209740 antibody
      • OTTHUMP00000209741 antibody
      • OTTHUMP00000209742 antibody
      • OTTHUMP00000209743 antibody
      • OTTHUMP00000209744 antibody
      • p40 antibody
      • p51 antibody
      • P51/P63 antibody
      • p53-related protein p63 antibody
      • p53CP antibody
      • p63 antibody
      • P63_HUMAN antibody
      • p73H antibody
      • p73L antibody
      • RHS antibody
      • SHFM4 antibody
      • TAp63alpha antibody
      • TP53CP antibody
      • TP53L antibody
      • TP63 antibody
      • TP73L antibody
      • Transformation related protein 63 antibody
      • Transformation-related protein 63 antibody
      • Trp53rp1 antibody
      • Trp63 antibody
      • Tumor protein 63 antibody
      • Tumor protein p53-competing protein antibody
      • Tumor protein p53-like antibody
      • Tumor protein p63 antibody
      • Tumor protein p63 deltaN isoform delta antibody
      • Tumor protein p73 antibody
      • Tumor protein p73-like antibody
      see all


    • Anti-p63 antibody [Y289] (ab32353) at 1/4000 dilution (unpurified) + A431 cell lysate at 10 µg

      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 77 kDa
      Observed band size: 73 kDa
      why is the actual band size different from the predicted?

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling p63 with purified ab32353
      at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-p63 antibody [Y289] (ab32353) at 1/10000 dilution (purified) + A431 cell lysate at 10 µg

      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 77 kDa
      Observed band size: 73 kDa why is the actual band size different from the predicted?

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular skin tissue labelling p63 with purified ab32353 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunocytochemsitry/Immunofluorescence analysis of A431 cells labelling p63 (red) with purified ab32353 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling p63 with unpurified ab32353 at 1/250 dilution.


    This product has been referenced in:
    • Firth AL  et al. Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells. Proc Natl Acad Sci U S A 111:E1723-30 (2014). Read more (PubMed: 24706852) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence
    Human Cell (Corneal limbal epithelial cells)
    Corneal limbal epithelial cells
    Yes - Triton 0.3% - 5 minutes

    Mr. Manuel Chacon

    Verified customer

    Submitted Dec 18 2013


    Thank you for contacting us.

    Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Abpromise guarantee:

    We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More


    Thank you for contacting us. I can provide the range in which the epitope is found. The peptide maps between
    amino acids 650 and 680 of human p63 (p73L) (UniProt: Q9H3D4), on the C-terminus. Please let me know if you have any questions.
    I hope this is helpful. Please contact me again if you have any further questions.

    Read More


    Sign up