Key features and details
- Rabbit polyclonal to p63RhoGEF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p63RhoGEF antibody
DescriptionRabbit polyclonal to p63RhoGEF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human p63RhoGEF aa 226-446.
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human breast carcinoma tissue.
Keep as concentrated solution.
Protein previously labeled a GEFT.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 64 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
Tissue specificityIsoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
DomainThe guanine nucleotide exchange activity is autoinhibited by the PH domain.
Cellular localizationCell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.
- Information by UniProt
- ARHGEF25 antibody
- ARHGP_HUMAN antibody
- GEFT antibody
ab127690 has been referenced in 2 publications.
- Wang Y et al. GEFT protein expression in digestive tract malignant tumors and its clinical significance. Oncol Lett 18:5577-5590 (2019). PubMed: 31620201
- Liu C et al. Epigenetically upregulated GEFT-derived invasion and metastasis of rhabdomyosarcoma via epithelial mesenchymal transition promoted by the Rac1/Cdc42-PAK signalling pathway. EBioMedicine 50:122-134 (2019). PubMed: 31761617