Overview

  • Product name
  • Description
    Rabbit polyclonal to p66 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 137-150 of Mouse p66 alpha .

  • Positive control
    • HeLa or Raw whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.

Target

  • Function
    Transcriptional repressor.
  • Tissue specificity
    Ubiquitous, both in fetal and adult tissues.
  • Sequence similarities
    Contains 1 GATA-type zinc finger.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20085 antibody
    • FLJ21017 antibody
    • GATA zinc finger domain containing 2A antibody
    • GATA zinc finger domain containing protein 2A antibody
    • GATA zinc finger domain-containing protein 2A antibody
    • GATAD2A antibody
    • Hp66 alpha antibody
    • Hp66alpha antibody
    • OTTHUMP00000201911 antibody
    • OTTHUMP00000201912 antibody
    • P66 alpha antibody
    • P66A_HUMAN antibody
    • p66alpha antibody
    • Transcriptional repressor p66 alpha antibody
    • Transcriptional repressor p66-alpha antibody
    • Transcriptional repressor p66alpha antibody
    see all

Images

  • Western blot analysis of p66a in 15 ugs of HeLa cell lysate using ab13714 at 1/500 dilution.
    Western blot analysis of p66a in 15 ugs of HeLa cell lysate using ab13714 at 1/500 dilution.

References

This product has been referenced in:
  • Mastroeni D  et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 31:2025-37 (2010). IHC-Fr ; Human . Read more (PubMed: 19117641) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for calling Abcam earlier today.

I am sorry about all of the problems that you are having with some of our products. As we discussed in the phone, I am sending you a new vial of ab1416 and the order number for that is ********.

As for ab13714, if the protocol variations we talked about do not prove to be successful, then please let me know as under out Abpromise we do guarantee it to work in WB on mouse and human samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

Please let me know if there is anything else I can help you with.

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Answer

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab13713 and ab13714. Both antibodies have been characterized for use in Western blotting with human and mouse samples, and HeLa or Raw whole cell lysate is recommended as positive controls. Both antibodies have not yet been tested for application in IP. If you could kindly provide some additional details regarding your protocol, I can better assist you with trouble-shooting. Below I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. Thank you, and I look forward to hearing from you soon. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=13713&mode=questionaire

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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