Anti-P70 S6 Kinase beta / SRK antibody (ab70271)

Overview

  • Product name
    Anti-P70 S6 Kinase beta / SRK antibody
    See all P70 S6 Kinase beta / SRK primary antibodies
  • Description
    Rabbit polyclonal to P70 S6 Kinase beta / SRK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human P70 S6 Kinase beta/ SRK aa 430-482 (C terminal). The exact sequence is proprietary.
    Database link: NP_003943.2

  • Positive control
    • HeLa whole cell lysate
  • General notes

    Previously labelled as P70 S6 Kinase beta. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-6 µg/mg of lysate.

Target

  • Function
    Phosphorylates specifically ribosomal protein S6.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Images

  • All lanes : Anti-P70 S6 Kinase beta / SRK antibody (ab70271) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 15 µg
    Lane 2 : HeLa whole cell lysate at 5 µg

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 35 kDa, 67 kDa, 69 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References

ab70271 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for the additional protocol details and for the image provided. Have you tried any shorter incubations with the primary, such as 1 hr? Although we normally recommend ON for the strongest signal, reducing this time may eliminate some of the ba...

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