Anti-P70 S6 Kinase beta/SRK antibody (ab131526)
Key features and details
- Rabbit polyclonal to P70 S6 Kinase beta/SRK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P70 S6 Kinase beta/SRK antibody
See all P70 S6 Kinase beta/SRK primary antibodies -
Description
Rabbit polyclonal to P70 S6 Kinase beta/SRK -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human P70 S6 Kinase beta/SRK aa 387-391 conjugated to keyhole limpet haemocyanin.
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Positive control
- Methanol-fixed HeLa cells, 293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 59 kDa).
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 59 kDa). |
ICC/IF
1/100 - 1/200. |
Target
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Function
Phosphorylates specifically ribosomal protein S6. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 6199 Human
- Omim: 608939 Human
- SwissProt: Q9UBS0 Human
- Unigene: 534345 Human
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Alternative names
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 2.7.11.1 antibody
- KS6B2_HUMAN antibody
see all
Images
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All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab131526) at 1/500 dilution
Lane 1 : 293 cell lysate with blocking peptide
Lane 2 : 293 cell lysate
Predicted band size: 59 kDa
Observed band size: 70,85 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131526 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab131526 has been referenced in 4 publications.
- Hao L et al. Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer. Aging (Albany NY) 12:2156-2168 (2020). PubMed: 32018225
- Wang B et al. Butorphanol Inhibits the Malignant Biological Behaviors of Ovarian Cancer Cells via Down-Regulating the Expression of TMEFF1. Onco Targets Ther 13:10973-10981 (2020). PubMed: 33149612
- He J & Zhu J Collapsin Response Mediator Protein-2 Ameliorates Sevoflurane-Mediated Neurocyte Injury by Targeting PI3K-mTOR-S6K Pathway. Med Sci Monit 24:4982-4991 (2018). PubMed: 30018280
- Li X et al. Atorvastatin regulates pericardial patch healing via the microRNA140-ADAM10-ephrinB2 pathway. Am J Transl Res 10:4054-4064 (2018). PubMed: 30662650