Key features and details
- Rabbit polyclonal to P70 S6 Kinase beta/SRK
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-P70 S6 Kinase beta/SRK antibody
See all P70 S6 Kinase beta/SRK primary antibodies
DescriptionRabbit polyclonal to P70 S6 Kinase beta/SRK
ab131526 detects endogenous levels of total P70 S6 Kinase beta/SRK.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human P70 S6 Kinase beta/SRK aa 387-391 conjugated to keyhole limpet haemocyanin.
- Methanol-fixed HeLa cells, 293 cell lysate
Previously labelled as P70 S6 Kinase beta.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 59 kDa).|
|ICC/IF||1/100 - 1/200.|
FunctionPhosphorylates specifically ribosomal protein S6.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 220.127.116.11 antibody
- KS6B2_HUMAN antibody
All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab131526) at 1/500 dilution
Lane 1 : 293 cell lysate with blocking peptide
Lane 2 : 293 cell lysate
Predicted band size: 59 kDa
Observed band size: 70,85 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131526 at 1/100 dilution.
ab131526 has been referenced in 2 publications.
- He J & Zhu J Collapsin Response Mediator Protein-2 Ameliorates Sevoflurane-Mediated Neurocyte Injury by Targeting PI3K-mTOR-S6K Pathway. Med Sci Monit 24:4982-4991 (2018). PubMed: 30018280
- Li X et al. Atorvastatin regulates pericardial patch healing via the microRNA140-ADAM10-ephrinB2 pathway. Am J Transl Res 10:4054-4064 (2018). PubMed: 30662650