Overview

  • Product name

    Anti-P70 S6 Kinase beta/SRK antibody
    See all P70 S6 Kinase beta/SRK primary antibodies
  • Description

    Rabbit polyclonal to P70 S6 Kinase beta/SRK
  • Host species

    Rabbit
  • Specificity

    ab131526 detects endogenous levels of total P70 S6 Kinase beta/SRK.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human P70 S6 Kinase beta/SRK aa 387-391 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Methanol-fixed HeLa cells, 293 cell lysate
  • General notes

    Previously labelled as P70 S6 Kinase beta. 

Properties

Applications

Our Abpromise guarantee covers the use of ab131526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/100 - 1/200.

Target

  • Function

    Phosphorylates specifically ribosomal protein S6.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Images

  • All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab131526) at 1/500 dilution

    Lane 1 : 293 cell lysate with blocking peptide
    Lane 2 : 293 cell lysate

    Predicted band size: 59 kDa
    Observed band size: 70,85 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131526 at 1/100 dilution.

References

This product has been referenced in:

  • He J & Zhu J Collapsin Response Mediator Protein-2 Ameliorates Sevoflurane-Mediated Neurocyte Injury by Targeting PI3K-mTOR-S6K Pathway. Med Sci Monit 24:4982-4991 (2018). Read more (PubMed: 30018280) »
  • Li X  et al. Atorvastatin regulates pericardial patch healing via the microRNA140-ADAM10-ephrinB2 pathway. Am J Transl Res 10:4054-4064 (2018). Read more (PubMed: 30662650) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer



In your first western blot it seems the doublet just above 50kDa marker is P70 S6 Kinase beta. The higher band of the doublet is predicted to be the phosphorylated form of the protein.

It might be nice to have a negative control, which doesn't express P70 S6 Kinase beta, if a cell line or tissue type like that exists.

Another idea is to treat a sample with phosphatase prior to loading the gel. The phosphatase treated samples should have no reactivity with the phospho-specific antibody.

Read More

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab131526.

This antibody is currently backordered. Expected delivery date is September 6.To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question
Answer

Thanks so much for including the molecular weight markers.

Looking at the blot again it does appear that the vial of ab131256 is not working as expected as its producing many non-specific bands. If you have purchased this product within the last 6 months or soI am happy to offer a replacement of any primary antibody, credit or refund.

In your reply, please let me know the PO number or Abcam order reference number associated with this order so I can process your request.

Read More

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