Overview

  • Product name

    Anti-P70 S6 Kinase beta/SRK antibody
    See all P70 S6 Kinase beta/SRK primary antibodies
  • Description

    Rabbit polyclonal to P70 S6 Kinase beta/SRK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human P70 S6 Kinase beta/SRK (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9UBS0

  • Positive control

    • WB: Mouse brain lysate. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as P70 S6 Kinase beta. 

Properties

Applications

Our Abpromise guarantee covers the use of ab223615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF 1/50.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Phosphorylates specifically ribosomal protein S6.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Images

  • 2% formaledehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P70 S6 Kinase beta/SRK (yellow) using ab223615 at 1/50 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.

    (A) DAPI (blue) nuclear stain. (B) ab223615. (C) Merge.

  • P70 S6 Kinase beta/SRK was immunoprecipitated from mouse brain lysate with ab223615 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab223615 at 1/1000 dilution.

    Lane 1: IP negative control

    Lane 2: ab223615 IP in mouse brain lysate.

  • 2% formaledehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P70 S6 Kinase beta/SRK (green) using ab223615 at 1/50 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI (blue) nuclear stain. (B) ab223615. (C) Merge.

References

ab223615 has not yet been referenced specifically in any publications.

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