Key features and details
- Rabbit polyclonal to P70 S6 Kinase beta/SRK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P70 S6 Kinase beta/SRK antibody
See all P70 S6 Kinase beta/SRK primary antibodies
DescriptionRabbit polyclonal to P70 S6 Kinase beta/SRK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
Synthetic peptide within Human P70 S6 Kinase beta/SRK aa 430-482 (C terminal). The exact sequence is proprietary.
Database link: NP_003943.2
- HeLa whole cell lysate
Previously labelled as P70 S6 Kinase beta.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 2-6 µg/mg of lysate.|
FunctionPhosphorylates specifically ribosomal protein S6.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 220.127.116.11 antibody
- KS6B2_HUMAN antibody
All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab70271) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 35 kDa, 67 kDa, 69 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
ab70271 has not yet been referenced specifically in any publications.