Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

Overview

  • Product name

    Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal
    See all P70 S6 Kinase beta/SRK primary antibodies
  • Description

    Rabbit monoclonal [EPR14956] to P70 S6 Kinase beta/SRK - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human P70 S6 Kinase beta/SRK aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBS0

  • Positive control

    • Human fetal liver lysate, Jurkat and K562 cell lysates, Human pancreas tissue. HeLa cells.
  • General notes

     

     

    Previously labelled as P70 S6 Kinase beta. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50.
ICC/IF 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Phosphorylates specifically ribosomal protein S6.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: RPS6KB2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab184551 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab184551 was shown to specifically react with P70 S6 Kinase beta/SRK in wild-type HAP1 cells. No band was observed when RPS6KB2 knockout samples were used. Wild-type and RPS6KB2 knockout samples were subjected to SDS-PAGE.  Ab184551 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution

  • Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/50 dilution, counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • All lanes : Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

References

This product has been referenced in:

  • Liu Y  et al. The overexpression of Rab9 promotes tumor progression regulated by XBP1 in breast cancer. Onco Targets Ther 12:1815-1824 (2019). Read more (PubMed: 30881034) »
  • Li J  et al. miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice. Biomed Res Int 2019:1528278 (2019). Read more (PubMed: 31355248) »
See all 2 Publications for this product

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