Product nameAnti-p73 alpha+beta antibody [ER-15]
DescriptionMouse monoclonal [ER-15] to p73 alpha+beta
Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human, Monkey
Recombinant fragment, corresponding to amino acids 380-637 of Monkey p73 alpha+beta.
- COS monkey kidney cells and IM9 human myeloma cells
This antibody does not cross-react with p53 or p53-related p51/Ket protein. This antibody binds to both p73a and p73b in immunoprecipitation and Western Blot assays.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
Purification notesPurified by Ammonium sulfate precipitation.
Primary antibody notesThis antibody does not cross-react with p53 or p53-related p51/Ket protein. This antibody binds to both p73a and p73b in immunoprecipitation and Western Blot assays.
Our Abpromise guarantee covers the use of ab17230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 17079275|
|IP||Use a concentration of 2 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 70-80 kDa.|
|EMSA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceThe p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. This antibody does not cross-react with p53 or p53-related p51/Ket protein. p73 is expressed in either a full-length form MW ~80kDa or a shorter MW ~70kDa mRNA variant, with exon 13 spliced out. This antibody binds to both p73a and p73b in immunoprecipitation and Western Blot assays. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53-dependent cell cycle arrest.
- p53 like transcription factor antibody
- p53 related protein antibody
- P73 antibody
Overlay histogram showing HEK293 cells stained with ab17230 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17230, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Chapeau EA et al. Resistance mechanisms to TP53-MDM2 inhibition identified by in vivo piggyBac transposon mutagenesis screen in an Arf-/-mouse model. Proc Natl Acad Sci U S A 114:3151-3156 (2017). Read more (PubMed: 28265066) »
- Sakil HAM et al. ?Np73 regulates the expression of the multidrug-resistance genes ABCB1 and ABCB5 in breast cancer and melanoma cells - a short report. Cell Oncol (Dordr) 40:631-638 (2017). WB ; Human . Read more (PubMed: 28677036) »