Overview

  • Product name

  • Description

    Rabbit polyclonal to p73
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 11-407 of Human p73 (Uniprot ID: O15350).

  • Positive control

    • Jurkat, 293T, A431 and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tiisue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • Tissue specificity

    Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • Sequence similarities

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain

    Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • Post-translational
    modifications

    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

Images

  • Anti-p73 antibody (ab137797) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 69 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling p73 with ab137797 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labelling p73 with ab137797 at 1/100 dilution.

References

This product has been referenced in:

  • Yoon MK  et al. Cytoplasmic pro-apoptotic function of the tumor suppressor p73 is mediated through a modified mode of recognition of the anti-apoptotic regulator Bcl-XL. J Biol Chem 293:19546-19558 (2018). Read more (PubMed: 30429221) »
See 1 Publication for this product

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