Anti-p73 antibody (ab137797)
Key features and details
- Rabbit polyclonal to p73
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-p73 antibody
See all p73 primary antibodies -
Description
Rabbit polyclonal to p73 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 11-407 of Human p73 (Uniprot ID: O15350).
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Positive control
- Jurkat, 293T, A431 and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tiisue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 69 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 69 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. -
Tissue specificity
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. -
Sequence similarities
Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsIsoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Accumulates in the nucleus in response to DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 7161 Human
- Entrez Gene: 362675 Rat
- Omim: 601990 Human
- SwissProt: O15350 Human
- Unigene: 697294 Human
- Unigene: 706990 Human
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Alternative names
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
see all
Images
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Anti-p73 antibody (ab137797) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 69 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling p73 with ab137797 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labelling p73 with ab137797 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137797 has been referenced in 4 publications.
- Zhang H et al. CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSa/ATM-p73 axis in bladder cancer. Mol Cancer 20:70 (2021). PubMed: 33874956
- Xie M et al. Secondary ossification center induces and protects growth plate structure. Elife 9:N/A (2020). PubMed: 33063669
- Yoon MK et al. Cytoplasmic pro-apoptotic function of the tumor suppressor p73 is mediated through a modified mode of recognition of the anti-apoptotic regulator Bcl-XL. J Biol Chem 293:19546-19558 (2018). PubMed: 30429221
- Wang X et al. Zoledronic acid inhibits the pentose phosphate pathway through attenuating the Ras-TAp73-G6PD axis in bladder cancer cells. Mol Med Rep 12:4620-4625 (2015). PubMed: 26126921