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    p73-antibody-ep436y-ab40658.pdf

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RecombinantRabMAb

Recombinant Anti-p73 antibody [EP436Y] (ab40658)

  • Datasheet
Reviews (1)Q&A (2)References (52)

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Western blot - Anti-p73 antibody [EP436Y] (ab40658)
  • Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EP436Y] (ab40658)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
  • Flow Cytometry (Intracellular) - Anti-p73 antibody [EP436Y] (ab40658)
  • Western blot - Anti-p73 antibody [EP436Y] (ab40658)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
  • Western blot - Anti-p73 antibody [EP436Y] (ab40658)
  • Anti-p73 antibody [EP436Y] (ab40658)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP436Y] to p73
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-p73 antibody [EP436Y]
    See all p73 primary antibodies
  • Description

    Rabbit monoclonal [EP436Y] to p73
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HEK293 and NIH/3T3 cell lysates. IHC-P: Human urinary bladder carcinoma, human kidney, human liver carcinoma and mouse testis tissues. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP436Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-p73 antibody [EP436Y] (ab197039)
    • HRP Anti-p73 antibody [EP436Y] (ab197040)
    • Anti-p73 antibody [EP436Y] - BSA and Azide free (ab219594)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • HEK-293 whole cell lysate (ab7902)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
1/150 - 1/300.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
WB
1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
1/150 - 1/300.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for IP.

Target

  • Function

    Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • Tissue specificity

    Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • Sequence similarities

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain

    Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • Post-translational
    modifications

    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Target information above from: UniProt accession O15350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7161 Human
    • Entrez Gene: 22062 Mouse
    • Omim: 601990 Human
    • SwissProt: O15350 Human
    • SwissProt: Q9JJP2 Mouse
    • Unigene: 697294 Human
    • Unigene: 706990 Human
    • Unigene: 78015 Mouse
    • Alternative names

      • p53 like transcription factor antibody
      • p53 related protein antibody
      • p53-like transcription factor antibody
      • p53-related protein antibody
      • p73 antibody
      • P73_HUMAN antibody
      • TP73 antibody
      • Tumor protein p73 antibody
      see all

    Images

    • Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/5000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HEK293 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 70 kDa
      Observed band size: 60,75 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EP436Y] (ab40658)
      Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EP436Y] (ab40658)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling p73 with purified ab40658 at a dilution of 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Flow Cytometry (Intracellular) - Anti-p73 antibody [EP436Y] (ab40658)
      Flow Cytometry (Intracellular) - Anti-p73 antibody [EP436Y] (ab40658)

      Intracellular Flow Cytometry analysis of 293 (human embryonic kidney) cells labeling p73 with purified ab40658 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

       

       

    • Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      Anti-p73 antibody [EP436Y] (ab40658) at 1/1000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 70 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder carcinoma tissue labelling p73 with unpurified ab40658 at a dilution of 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      Western blot - Anti-p73 antibody [EP436Y] (ab40658)
      All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/2000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa
      Observed band size: 63 kDa why is the actual band size different from the predicted?

    • Anti-p73 antibody [EP436Y] (ab40658)
      Anti-p73 antibody [EP436Y] (ab40658)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (52)

    Publishing research using ab40658? Please let us know so that we can cite the reference in this datasheet.

    ab40658 has been referenced in 52 publications.

    • Malik N  et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. PLoS Genet 17:e1009553 (2021). PubMed: 33945523
    • Sadžak A  et al. Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. Molecules 26:N/A (2021). PubMed: 33562817
    • Sari AN  et al. Identification and Characterization of MortaparibPlus-A Novel Triazole Derivative That Targets Mortalin-p53 Interaction and Inhibits Cancer-Cell Proliferation by Wild-Type p53-Dependent and -Independent Mechanisms. Cancers (Basel) 13:N/A (2021). PubMed: 33671256
    • Marshall CB  et al. Tissue-specific expression of p73 and p63 isoforms in human tissues. Cell Death Dis 12:745 (2021). PubMed: 34315849
    • Zhao H  et al. Targeted Inhibition of the E3 Ligase SCFSkp2/Cks1 Has Antitumor Activity in RB1-Deficient Human and Mouse Small-Cell Lung Cancer. Cancer Res 80:2355-2367 (2020). PubMed: 32265224
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-p73 antibody [EP436Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (hairy skin sections)
    Specification
    hairy skin sections
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TRITON, 3'
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 07 2011

    Question

    I would very much like to use this antibody for IP for a ChIP experiment. Could you tell me why it is considered "unsuitable".

    Read More

    Abcam community

    Verified customer

    Asked on Aug 02 2013

    Answer

    ab40658 was tested in IP on 293T lysate, and detected a faint smear along with two non-specific bands. As a result, we didn't recommend it for use in IP.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Aug 02 2013

    Question

    One of our customers asks id there is cross reactivity with the delta-n isoform of the p73 protein

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2011

    Answer

    The p73 antibody ab40658 should recognize all p73 isoforms, including the delta-n isoforms, except for isoform 10 according to the uniprot file below: http://www.uniprot.org/uniprot/O15350

    Read More

    Abcam Scientific Support

    Answered on Nov 01 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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