Product nameAnti-p75 NGF Receptor antibody [5C4B9]
See all p75 NGF Receptor primary antibodies
DescriptionMouse monoclonal [5C4B9] to p75 NGF Receptor
SpecificityPlease note that, despite having used an immunogen that is 100% homologous with the human and rat p75 NGF receptor proteins, Abcam could not get this antibody to detect p75 NGF receptor in human or rat tissue lysates. Abcam would welcome feedback from customers that have used ab86768 in either of these species!
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide corresponding to Human p75 NGF Receptor aa 250-350.
- This antibody gave a positive signal in the following mouse tissue lysates: Brain; Spinal Cord; Heart.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab86768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 75 kDa).|
FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainDeath domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.
- Information by UniProt
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
All lanes : Anti-p75 NGF Receptor antibody [5C4B9] (ab86768) at 5 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Please note that, despite having used an immunogen that is 100% homologous with the human and rat p75 NGF receptor proteins, Abcam could not get this antibody to detect p75 NGF receptor in human or rat tissue lysates. Abcam would welcome feedback from customers that have used ab86768 in either of these species!
ab86768 has not yet been referenced specifically in any publications.