Anti-p75 NGF Receptor antibody [8J2] (ab245134)
Key features and details
- Mouse monoclonal [8J2] to p75 NGF Receptor
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
-
Product name
Anti-p75 NGF Receptor antibody [8J2]
See all p75 NGF Receptor primary antibodies -
Description
Mouse monoclonal [8J2] to p75 NGF Receptor -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human p75 NGF Receptor aa 1-250 (extracellular).
Database link: P08138 -
Positive control
- ICC/IF: C6 cells. Flow Cyt: SH-SY5Y and C6 cells. IP: p75 NGF Receptor IP in C6 cell lysate. WB: Mouse p75 NGF Receptor-transfected cells. SH-SY5Y and C6 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute with 100 microlitre sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituents: PBS, 3% Trehalose
pH 7.2 - 7.6 -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
8J2 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa.
Non-reducing conditions only - no DTT or beta-mercapoethanol. |
|
Flow Cyt |
Use a concentration of 5 - 20 µg/ml.
|
|
ICC/IF |
Use a concentration of 1 - 5 µg/ml.
Light fixation only or unfixed works best. Epitope is sensitive to fixation. |
|
IP |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa. Non-reducing conditions only - no DTT or beta-mercapoethanol. |
Flow Cyt
Use a concentration of 5 - 20 µg/ml. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. Light fixation only or unfixed works best. Epitope is sensitive to fixation. |
IP
Use a concentration of 1 µg/ml. |
Target
-
Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
- SwissProt: P07174 Rat
- Unigene: 415768 Human
see all -
Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
-
Non-fixed and 4% formaldehyde-fixed C6 (rat glial tumor cell line) cells labeling p75 NGF Receptor using ab245134 at 2 µg/ml for 1 hour (green) in ICC/IF.
Blocking: 10% normal horse serum, 30 mins.
Secondary antibody: Donkey anti-mouse-CF488A at 2 µg/mL for 1 hour.
Nuclei stained with Hoechst (blue).
-
Flow Cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) (Left panel) and C6 (rat glial tumor cell line) (Right panel) staining p75 NGF Receptor using ab245134 (red lines) and a different antibody (solid black lines) at 2 µg per 106 cells, 60 mins on ice. Negative control is the dashed line, Non specific Control IgG.
Blocking: 200 µg/mL sheep IgG, 30 minutes on ice.
Secondary antibody: Goat anti-mouse-PE used at a 1/100 dilution for 20 mins in the dark on ice.
-
Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134) (10 µg antibody per 200 µg total protein):
Lane 1: p75 NGF Receptor IP in C6 (rat glial tumor cell line) whole cell lysate.
Lane 2: Control Protein G agarose (10 µL).
Precipitated p75 NGF Receptor was eluted off the beads and antibody by heating and addition of SDS-PAGE sample buffer.
WB method: ab245134 (1 µg/mL) and secondary anti-sheep-HRP (1/6000) antibody.
Detection: Chemiluminiscence.
-
All lanes : Anti-p75 NGF Receptor antibody [8J2] (ab245134) at 1 µg/ml (overnight at 4°C)
Lane 1 : Mouse p75 NGF Receptor-transfected cells
Lane 2 : Non-transfected control cells
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 4 : C6 (rat glial tumor cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : anti-mouse-HRP, 1 hour at RT at 1/6000 dilution
Performed under non-reducing conditions.
Predicted band size: 45 kDaSDS-PAGE: 4-12%, non-reducing conditions.
Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 µm).
Detection: Chemiluminiscence.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab245134 has been referenced in 1 publication.
- Yuan H et al. A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development. Front Physiol 13:981311 (2022). PubMed: 36213234