Anti-p75 NGF Receptor antibody (ab216495)
Key features and details
- Rabbit polyclonal to p75 NGF Receptor
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p75 NGF Receptor antibody
See all p75 NGF Receptor primary antibodies -
Description
Rabbit polyclonal to p75 NGF Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Rat p75 NGF Receptor aa 250-350 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P07174 -
Positive control
- Rat liver and brain tissues; Mouse brain lysate; HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
IHC-P |
1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody). |
|
WB |
1/100 - 1/1000. Predicted molecular weight: 45 kDa.
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|
Flow Cyt |
1/100.
|
Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/100 - 1/500. (or 1/50 - 1/200 if using a fluorescent secondary antibody). |
WB
1/100 - 1/1000. Predicted molecular weight: 45 kDa. |
Flow Cyt
1/100. |
Target
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Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
- SwissProt: P07174 Rat
- Unigene: 415768 Human
see all -
Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
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Flow Cytometry of Mouse Spleen with ab217628 at 1/100
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.
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Anti-p75 NGF Receptor antibody (ab216495) at 1/300 dilution + Mouse brain lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 45 kDa -
Immunofluorescent analysis of HepG2 cells labeling p75 NGF Receptor with ab216495 at 1/500 dilution in PBS (incubated for two hours at 37°C), followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216495 has not yet been referenced specifically in any publications.