Overview

  • Product name

    Anti-p75 NGF Receptor antibody
    See all p75 NGF Receptor primary antibodies
  • Description

    Rabbit polyclonal to p75 NGF Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Rat p75 NGF Receptor aa 287-337 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    NSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQTHTQTASGQALKGDGNL Y


    Database link: P07174

  • Positive control

    • Rat liver and brain tissues; Mouse brain lysate; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab216495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/100 - 1/500.

(or 1/50 - 1/200 if using a fluorescent secondary antibody).

WB 1/100 - 1/1000. Predicted molecular weight: 45 kDa.
Flow Cyt 1/100.

Target

  • Function

    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain

    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications

    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Flow Cytometry of Mouse Spleen with ab217628 at 1/100
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.

  • Anti-p75 NGF Receptor antibody (ab216495) at 1/300 dilution + Mouse brain lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 45 kDa

  • Immunofluorescent analysis of HepG2 cells labeling p75 NGF Receptor with ab216495 at 1/500 dilution in PBS (incubated for two hours at 37°C), followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

References

ab216495 has not yet been referenced specifically in any publications.

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