Overview

  • Product name

    Anti-p75 NGF Receptor antibody
    See all p75 NGF Receptor primary antibodies
  • Description

    Rabbit polyclonal to p75 NGF Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human p75 NGF Receptor (internal sequence). The exact sequence is proprietary.
    Database link: P08138

  • Positive control

    • WB: NTERA-2 cl.D1 [NT2/D1], PC-3 and U-87 MG whole cell lysates; PC-12 whole cell extract; Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue. IHC-Fr: Rat E13.5 brain tissue. ICC/IF: Culture rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab227509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain

    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications

    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Paraffin-embedded rat brain tissue stained for p75 NGF Receptor with ab227509 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution

    Lane 1 : NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    7.5% SDS-PAGE gel.

  • Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed rat E18 primary cortical neuron cells stained for p75 NGF Receptor (green) using ab227509 at 1/250 dilution in ICC/IF.

    Counterstain: Beta Tubulin 3/ TUJ1, stained with beta Tubulin 3/ TUJ1 antibody (red). Nuclear counterstain: Fluoroshield with DAPI (blue). 

  • Frozen section of rat E13.5 brain tissue stained for p75 NGF Receptor (green) using ab227509 at 1/250 dilution in immunohistochemical analysis.

    Counterstain: Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red). Nuclear counterstain: Fluroshield with DAPI (blue). 

References

ab227509 has not yet been referenced specifically in any publications.

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