Product nameAnti-p75 NGF Receptor antibody
See all p75 NGF Receptor primary antibodies
DescriptionGoat polyclonal to p75 NGF Receptor
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat
- WB: Human Duodenum and Human Heart lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAb32888 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab32888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 45 kDa). The observed molecular weight corresponds to earlier findings in literature (see reference provided).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainDeath domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.
- Information by UniProt
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
Anti-p75 NGF Receptor antibody (ab32888) at 0.3 µg/ml + Human Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
The observed molecular weight corresponds to earlier findings in the literature (see reference provided).
This product has been referenced in:
- Pham DD et al. p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3. J Biol Chem 291:10747-58 (2016). Read more (PubMed: 26984409) »
- Han HW et al. The Nogo-C2/Nogo receptor complex regulates the morphogenesis of zebrafish lateral line primordium through modulating the expression of dkk1b, a Wnt signal inhibitor. PLoS One 9:e86345 (2014). WB ; Zebrafish . Read more (PubMed: 24466042) »