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An extracellular fragment from the mouse p75 receptor (amino acids 43-161).
Our Abpromise guarantee covers the use of ab8874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/400. PubMed: 169737171/100.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab11382 staining normal mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 555 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary. This image (taken at 80x) demonstrates p75 positive neurons in the basal forebrain.
NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8874 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result).
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