Overview

  • Product name
    Anti-p75 NGF Receptor antibody
    See all p75 NGF Receptor primary antibodies
  • Description
    Rabbit polyclonal to p75 NGF Receptor
  • Host species
    Rabbit
  • Specificity
    This antibody recognises mouse NGF Receptor
  • Tested applications
    Suitable for: IHC-Fr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Mouse p75 NGF Receptor aa 43-161 (extracellular).

  • Positive control
    • WB: NIH/3T3 and WiDr cell lysate. Flow Cyt: Mouse neuroblastoma and rat glioma cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab8874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/400. PubMed: 169737171/100.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + NIH-3T3 cells at 10 µg

    Secondary
    HRP-conjugated Donkey anti-Rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes

    See Abreview

  • NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8874 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result). 

  • Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + whole cell lysate prepared from human WiDr colorectal cancer cell line at 5 µg

    Secondary
    HRP conjugated donkey anti-rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Exposure time: 12 minutes

    See Abreview

References

This product has been referenced in:
  • Sun X  et al. Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function. Stem Cell Res Ther 9:133 (2018). ICC/IF . Read more (PubMed: 29751848) »
  • Sparks NRL  et al. Low Osteogenic Yield in Human Pluripotent Stem Cells Associates with Differential Neural Crest Promoter Methylation. Stem Cells 36:349-362 (2018). Read more (PubMed: 29193426) »
See all 44 Publications for this product

Customer reviews and Q&As

1-10 of 26 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 18 2014

Answer

ab8874 was raised against a synthetic peptide containing a GST tag. However, ab8875 is the immunogen affinity purified version of ab8874 and was purified to remove all antibody that recognizes the GST tag, so only antibody recognizing p75 NGF receptor remains.
https://www.abcam.com/p75-ngf-receptor-antibody-ab8875.html

Alternatively, ab38335 is another anti-p75 NGFR antibody that has been immunogen affinity purified:
https://www.abcam.com/p75-ngf-receptor-antibody-me20-4-ab8877.html

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (nerve)
Loading amount
25 µg
Specification
nerve
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cell)
Specification
Mesenchymal stem cell
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Mr. Steven Johnstone

Verified customer

Submitted Sep 05 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab8874with the order number 1151618.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your enquiry.

I can confirm that ab8874 Anti-p75 NGF Receptor antibody is sold as whole antiserum.

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

Antibody concentration is usually determined by protein assay, and serum will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 -5mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for contacting us. I am sorry to hear that you are experiencing problems with this antibody.

The FACS protocol we have used for this antibody is very simple. Please find attached the document.

You may consider following steps for troubleshooting

- Using higher/ low concentration of primary antibody
- Incubating primary for 1-2 hours 4C
- Use WiDr or NIH3T3 as positive control

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

TThank you for calling Abcam regarding positive controls.

AB10404 has been used in IHC-P on lung sections in the following publication:Liu Yet al.Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment.J Virol83:6837-48 (2009).http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=19357175&dopt=Abstract.
This product has also been used by customers in human prostate carcinoma tissue to good effect. More information about this can be seen at this link:https://www.abcam.com/index.html?datasheet=10404&tab=abreviews&intabreviewid=5255

In regards to AB8874: The publication,Liu Set al.Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.Am J Pathol166:891-900 (2005).http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15743800&dopt=Abstract,uses human schwannoma as a positive control for anti-human p75NGFRantibody.

I hope that this information is helpful. Please let me know if you have any questions.

Read More

Answer

Thank you for providing that extra information. I now understanf more fully what the customer is trying to achieve and the problems they have been encountering.

As I mentioned in my previous email, the two lots used by the customer are from the same preparation of serum, they have simply been aliquotted at different times. You have clearly seen a marked difference in the activity of the two lots,it may be that the GR1525-8 lotsomehow lost some activity compared to GR1525-6 during theprocessing or delivery. This is uncommon and we have not had anysimilar complaints against this antibody.

I would be happy to replace the GR1525-8 vial with a different vial. The only alternative to GR1525-8we have in stock is GR1525-7. As I said previously, this is from the same stock but is prepared at a different time. If you would prefer, I could send you an alternative antibody to the same target, such as https://www.abcam.com/p75-NGF-Receptor-antibody-NGFR5-ab3125.html.

In order to organise this I will need the order number of the ab8874 (lot number GR1525-8).

I look forward to hearing how you would like to proceed.

Read More

Answer

Thank you for your call today and for letting us know about the trouble with this new vial of ab8874.

As we discussed, I'm sending a free of charge vial on the order ***, which should arrive tomorrow. Please keep me updated about the results using this new vial.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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