Overview

  • Product name
    Anti-p75 NGF Receptor antibody
    See all p75 NGF Receptor primary antibodies
  • Description
    Rabbit polyclonal to p75 NGF Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Mouse p75 NGF Receptor aa 43-161 (extracellular).

  • Positive control
    • Flow Cyt: NG6 cells. ICC/IF: NG6 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab8875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Paraformaldehyde-fixed, NG6 (fusion of a mouse neuroblastoma and rat glioblastoma) cells labeling p75 NGF Receptor (green) using ab8875 at 10 µg/ml in ICC/IF, followed by goat anti-rabbit-FITC secondary at 50 µg/ml. The nuclear stain is DAPI (blue).

  • NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8875 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result). 

  • ab8875 staining p75 NGF Receptor in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chang YT  et al. DNA methyltransferase inhibition restores erythropoietin production in fibrotic murine kidneys. J Clin Invest 126:721-31 (2016). Read more (PubMed: 26731474) »
  • Motohashi T  et al. Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells. Biol Open 5:311-22 (2016). Flow Cyt . Read more (PubMed: 26873953) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Sample
Rat Tissue sections (Plantar Skin)
Specification
Plantar Skin
Permeabilization
Yes - 10% Tx
Fixative
Paraformaldehyde

Mr. Matthew Thakur

Verified customer

Submitted Oct 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 22 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Embryonic lung)
Loading amount
30 µg
Specification
Embryonic lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 20 2012

Question
Answer

Thank you very much for your call yesterday.

I've checked with the lab, and ab8875 is provided in PBS only, so it is suitable to use with the EasyLink conjugation kits. I had another look at the recommended dimensions, and you are correct, the 3x10ug kit ab102885 will be sufficient for one vial of ab8875. However, with ab102885 you will need to run 3 conjugations (these can be done side-by-side) in order to conjugate the whole vial of ab8875. With ab102884, you can conjugate the full vial of ab8875 in one conjugation reaction, though you will have excess conjugation materials. Please let me know if you have any questions about this.

So, I've created a discount code which can be redeemed for 15% off any of our FITC EasyLink kits, so that you can choose which one will be best for you.

I hope that this information will be useful, but if you need anything else let me know and I'll be happy to help. Have a nice day!

Read More

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab8875 inbovine. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for bovine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab8874with the order number 1151618.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting Abcam regarding ab8875.


I have confirmed with the laboratory that our in-house quality control quantifies proteinby way of BCA. This method produced the 1.4 mg/ml concentration issued to this specific lot. Retesting has produced identical results.


I am sorry to hear that you are disappointed with the quantity of antibody received. I would be happy to send out a replacement vial free of charge as a courtesy. Please let me know if this would be of interest to you. I look forward to your reply.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for your enquiry and your patience. (1) It is dissolved in PBS and it does not contain any Tris or glycine or any preservatives. (2) 4.2 ug are used for each sample, which would have 1x10^6 cells. We did not directly label the primary antibody with a fluorochrome. A labeled secondary antibody was used, specifically FITC-labeled Goat-anti-rabbit.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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