Overview

  • Product name
    Anti-p75 NGF Receptor antibody [MC-192]
    See all p75 NGF Receptor primary antibodies
  • Description
    Mouse monoclonal [MC-192] to p75 NGF Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat PC-12 cells.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
  • General notes


    A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab6172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20213527

Target

  • Function
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Niapour N  et al. An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves. Cytotechnology 68:629-36 (2016). Read more (PubMed: 25680695) »
  • Nelson AR  et al. Noradrenergic sympathetic sprouting and cholinergic reinnervation maintains non-amyloidogenic processing of AßPP. J Alzheimers Dis 38:867-79 (2014). IHC ; Rat . Read more (PubMed: 24081376) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Cat Tissue sections (brain stem)
Specification
brain stem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem slice)
Specification
brainstem slice
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2014

Answer

Thank you very much for your interest in ab6172.

To our knowledge, ab6172 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab6172 now, test it inhuman and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab6172 in human. I will then send a discount code. This code must be issued before purchasing ab6172 so please wait for my reply before ordering.

2. Purchase ab6172 either by phone, fax, or online (www.abcam.com).

3. Test it in human.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6172 turns out to be unsuitable for human, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. I apologise for any confusion that has been caused with product Ab6172. There is a concentration value cited on the product data sheet, as 1.0mg/ml. You should have received a copy of this data sheet upon receipt of the product. I apologise if this was not the case, it is possible that the data sheet has only been recently updated with this information. Good luck with your research, if you need any further clarification please do not hesitate to get back in touch with me.

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Question
Answer

Dear Dr Hilker I am afraid that this antibody only recognises rat Low Affinity Nerve Growth Factor receptor. Regards Michael

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Answer

Thank you for your enquiry. This antibody is not currently available biotinylated, however we do offer a conjugation service for £150 pounds sterling or $225 US dollars. We would need to biotinylate at least 1mg antibody; the price listed on our web site (£187.50 pounds sterling or $282 US dollars) is for 0.1mg unlabelled antibody, so the total price of 1mg biotinylated antibody would be £2025 pounds sterling or $3045 US dollars.

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Question
Answer

Yes, this antibody is ideal for FACS of PC12 cells.

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Question
Answer

Yes, the antibody is excellent in these applications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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