Overview

  • Product name
    Anti-p75 NGF Receptor antibody [NGFR5]
    See all p75 NGF Receptor primary antibodies
  • Description
    Mouse monoclonal [NGFR5] to p75 NGF Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human, Ferret, Monkey
    Predicted to work with: Rabbit, Cat, BaboonDoes not react with: Mouse, Rat
  • Immunogen

    NGFR from A875 melanoma cells.

  • Epitope
    Amino acids 1 - 160.
  • Positive control
    • Melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab3125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
    • Sequence similarities
      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • Domain
      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.
    • Post-translational
      modifications
      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD271 antibody
      • CD271 antigen antibody
      • Gp80 LNGFR antibody
      • Gp80-LNGFR antibody
      • Low affinity nerve growth factor receptor antibody
      • Low affinity neurotrophin receptor p75NTR antibody
      • Low-affinity nerve growth factor receptor antibody
      • Nerve growth factor receptor antibody
      • Nerve growth factor receptor TNFR superfamily member 16 antibody
      • NGF receptor antibody
      • Ngfr antibody
      • p75 ICD antibody
      • p75 Neurotrophin receptor antibody
      • p75 NTR antibody
      • p75(NTR) antibody
      • p75NTR antibody
      • TNFR Superfamily Member 16 antibody
      • TNFRSF16 antibody
      • TNR16_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 16 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for p75 NGF Receptor using ab3125 at 1/50 dilution in immunohistochemical analysis.

      See Abreview

    • ICC/IF image of ab3125 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab3125 staining p75 NGF Receptor in Human cholinergic neurons by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in sodium citrate buffer prior to blocking with 2.5% serum for 1 hour 30 minutes at 21°C. The primary antibody was diluted 1/250 in normal horse serum blocking buffer and incubated with the sample for 65 hours at 4°C.

      See Abreview

    • ab3125 staining p75 NGF Receptor in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked for 1 hour at room temperature and then incubated with ab3125 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-mouse polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.

      See Abreview

    References

    This product has been referenced in:
    • Gong S  et al. The Therapeutic Effects after Transplantation of Whole-Layer Olfactory Mucosa in Rats with Optic Nerve Injury. Biomed Res Int 2018:6069756 (2018). Read more (PubMed: 29713644) »
    • Li X  et al. Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1. Oncol Lett 15:7097-7103 (2018). Read more (PubMed: 29731875) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-10 of 14 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (neural precursor)
    Permeabilization
    No
    Specification
    neural precursor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Sep 13 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Spermatogonial stem cells differentiating into gli)
    Permeabilization
    Yes - 0.1 % Triton-X
    Specification
    Spermatogonial stem cells differentiating into gli
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Dominic Schomberg

    Verified customer

    Submitted Apr 18 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Bone Marrow)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Specification
    Bone Marrow
    Blocking step
    Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    10% NBF, 10% Formic Acid Decalcification

    Premier Laboratory

    Verified customer

    Submitted Mar 29 2016

    Answer

    We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the datasheets. (Human, ferret, and monkey are listed on the ab3125 datasheet and human, mouse, rat and several others on the ab12228 datasheet).

    Note that metallothionein is cytoplasmic, so you will not be able to sort live cells. Antibody ab3125 anti-p75 NGF Receptor, on the other hand, recognizes an extracellular epitope, so you should be able to sort live cells.

    Read More

    Answer

    Merci pour votre émail. Je suis désolée que nous pouvons par regarder les images du coup. Nous sommes en Angleterre, et je pense que ça va devenir compliqué pour envoyer les lames.

    Je vais chercher pour trouver des images de mon coté et je vous tiens au courant. Est-ce que en attendant, vous pouvez me confirmerles pointssuivants:

    1.)que ces bien des tissus d'origine humaine que vous avez marqué? Ou c'est des tissus d'origine de souris?

    2.) C'est quoi comme sérum que vous avez utilisé pour bloquer les lames?

    3.) A votre avis, le problème vient de quel réactif?

    Je me réjouis de votre réponse. Veuillez s'il vous plait aussi me confirmer comment vous souhaitez procéder.

    Read More

    Answer

    Thank you for your inquiry.

    I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mouse monoclonal ab8877 (as can be seen on each datasheet in the section 'Alternative names'):
    Click here (or use the following: https://www.abcam.com/index.html?datasheet=8874).
    Click here (or use the following: https://www.abcam.com/index.html?datasheet=8877).

    These antibodies are guaranteed to work with mouse and human samples (amongst others) and in a number of applications. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

    If you are looking for other specifications (e.g. other species or applications), I would be pleased to assist you further in finding the appropriate antibody.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

    We do not have many qPCR primer in our catalog. We have primer for common controls such as GAPDH (ab83588) available, but CD271/p75-NGF receptor is unfortunately not in this selection.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Melanoma)
    Specification
    Melanoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
    Permeabilization
    Yes - 0.25%Triton X-100
    Blocking step
    DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

    Hongwei Shao

    Verified customer

    Submitted Nov 09 2011

    Answer

    Le tissu cérébral humain est en effet un tissu de choix pour l'expression de TrkA, NGF et le récepteur p75 NGF. Avez-vous reçu ces 3 anticorps en même temps (même colis de livraison)?  

    Read More

    Answer

    Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3125, ab6199, ab37837 en IHC-P. Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 6 mois, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir. J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. - Pour les 3 anticorps je recommanderais une incubation sur la nuit à 4°C (ou même à température ambiante) en prenant soin que les échantillons ne soient pas sujet à évaporation. Pour les dilutions des anticorps primaires, se reporter aux fiches techniques. - Pour les 3 anticorps je recommanderais également un démasquage de l'antigène à haute température en utilisant du tampon Tris EDTA pH 9,0. Pourriez-vous me communiquer quelques détails concernant la préparation des échantillons (agent et temps de fixation)? Les anticorps secondaires sont-il compatibles? Sont-ils bons? J'espère que ces informations vous aideront. Si les suggestions ne permettent pas d'améliorer vos résultats, n'hésitez pas à me recontacter pour plus d'assistance.

    Read More

    1-10 of 14 Abreviews or Q&A

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