Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
Key features and details
- Mouse monoclonal [NGFR5] to p75 NGF Receptor
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-p75 NGF Receptor antibody [NGFR5]
See all p75 NGF Receptor primary antibodies -
Description
Mouse monoclonal [NGFR5] to p75 NGF Receptor -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Cat, BaboonDoes not react with: Mouse -
Immunogen
Full length native protein (purified) corresponding to p75 NGF Receptor. Purified from A875 melanoma cell line.
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Epitope
Amino acids 1 - 160. -
Positive control
- IHC-P: Human testis tissue. ICC/IF: PC-12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 0.0268% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NGFR5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
- APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
- Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
- PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
- PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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IHC-P | (3) |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 101101519 Cat
- Entrez Gene: 4804 Human
- Entrez Gene: 100349095 Rabbit
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: P07174 Rat
- Unigene: 415768 Human
see all -
Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.
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ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab3125 has been referenced in 27 publications.
- Wang Y et al. A rare case of arrhythmogenic right ventricular cardiomyopathy associated with LAMA2 mutation: A case report and literature review. Front Med (Lausanne) 9:922347 (2022). PubMed: 35924034
- Lim JY et al. Potential application of human neural crest-derived nasal turbinate stem cells for the treatment of neuropathology and impaired cognition in models of Alzheimer's disease. Stem Cell Res Ther 12:402 (2021). PubMed: 34256823
- Li HX et al. Low-intensity extracorporeal shock wave therapy promotes recovery of sciatic nerve injury and the role of mechanical sensitive YAP/TAZ signaling pathway for nerve regeneration. Chin Med J (Engl) 134:2710-2720 (2021). PubMed: 34845995
- Zhang Y et al. Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration. Neural Regen Res 15:1903-1911 (2020). PubMed: 32246639
- Castle MJ et al. Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer's Disease Identifies a Need for Improved Vector Delivery. Hum Gene Ther 31:415-422 (2020). PubMed: 32126838