Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [NORI138B] to p75 NGF Receptor
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-p75 NGF Receptor antibody [NORI138B]
See all p75 NGF Receptor primary antibodies
DescriptionRat monoclonal [NORI138B] to p75 NGF Receptor
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein. This information is considered to be commercially sensitive.
- WB: Human brain, Neuro-2a, Mouse brain and Rat brain, PC-12 lysates. IHC-P: Human tonsil tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab271289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 45 kDa.|
FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainDeath domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.
- Information by UniProt
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling p75 NGF with ab271289 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the germinal center and basement membrane on the epithelium in the human tonsil. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0)
All lanes : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: lane 1, 2 : 3 minutes lane 3-5 : 59 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271289 has not yet been referenced specifically in any publications.