Western blot abreview for Anti-p8/NUPR1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human colon cancer cell)
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Loading amount
30 µg
Specification
human colon cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% NFDM
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 8-10 kDa

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Submitted Apr 09 2013

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