Overview

  • Product name
    Anti-p92 antibody - N-terminal
    See all p92 primary antibodies
  • Description
    Rabbit polyclonal to p92 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human p92 aa 183-212 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O75366

  • Positive control
    • K562 cell line lysate; Human colon carcinoma tissue; K562 cells.
  • General notes

    Previously labelled as Advillin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab175126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 92 kDa.

Target

  • Function
    Ca(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  • Tissue specificity
    Most highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection. Cell projection > axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein DOC 6 antibody
    • Actin binding protein DOC6 antibody
    • Advil antibody
    • Advillin antibody
    • AVIL antibody
    • AVIL_HUMAN antibody
    • DOC 6 antibody
    • DOC6 antibody
    • p92 antibody
    see all

Images

  • Anti-p92 antibody - N-terminal (ab175126) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 92 kDa

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling p92 with ab175126 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometrical analysis of K562 cells labeling p92 with ab175126 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175126.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up