Overview

  • Product name

    Anti-p95/NBS1 antibody [7E4C2]
    See all p95/NBS1 primary antibodies
  • Description

    Mouse monoclonal [7E4C2] to p95/NBS1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human p95/NBS1 aa 467-615. Expressed in E. Coli.
    Sequence:

    ERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDT NSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQL FKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKIS


    Database link: O60934

  • Positive control

    • Human p95/NBS1 recombinant protein; HEK293 cell lysate, transfected with p95/NBS1 (amino acids 467-615)-IgGFc; Jurkat cell lysate; HeLa cells; Human cervical cancer and colon cancer tissues.
  • General notes

     This product was previously labelled as p95 NBS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab181780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 85 kDa.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/200 - 1/1000.

Target

  • Function

    Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificity

    Ubiquitous. Expressed at high levels in testis.
  • Involvement in disease

    Nijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 FHA domain.
  • Domain

    The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    modifications

    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localization

    Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT V1 antibody
    • AT V2 antibody
    • ATV antibody
    • Cell cycle regulatory protein p95 antibody
    • FLJ10155 antibody
    • MGC87362 antibody
    • Nbn antibody
    • NBN_HUMAN antibody
    • NBS 1 antibody
    • NBS antibody
    • NBS1 antibody
    • Nibrin antibody
    • Nijmegen breakage syndrome 1 (nibrin) antibody
    • Nijmegen breakage syndrome antibody
    • Nijmegen breakage syndrome protein 1 antibody
    • p95 antibody
    • p95 protein of the MRE11/RAD50 complex antibody
    see all

Images

  • Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution + Human p95/NBS1 recombinant protein (amino acids 467-615)

    Predicted band size: 85 kDa

  • All lanes : Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with p95/NBS1 (amino acids 467-615)-hIgGFc

    Predicted band size: 85 kDa

  • Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution + Jurkat cell lysate

    Predicted band size: 85 kDa

  • Immunofluorescent analysis of HeLa cells labeling p95/NBS1 with ab181780 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow cytometric analysis of HeLa cells labeling p95/NBS1 with ab181780 at 1/200 dilution (green); negative control (red).

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling p95/NBS1 with ab181780 at 1/200 dilution followed by DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling p95/NBS1 with ab181780 at 1/200 dilution followed by DAB staining.

References

ab181780 has not yet been referenced specifically in any publications.

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