Anti-p95/NBS1 antibody (ab175800)
Key features and details
- Rabbit polyclonal to p95/NBS1
- Suitable for: IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-p95/NBS1 antibody
See all p95/NBS1 primary antibodies -
Description
Rabbit polyclonal to p95/NBS1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
General notes
This product was previously labelled as p95 NBS1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Constituent: Water -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ICC/IF |
1/1000.
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IHC-P |
1/1000.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
1/1000. |
IHC-P
1/1000. |
Target
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Function
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. -
Tissue specificity
Ubiquitous. Expressed at high levels in testis. -
Involvement in disease
Nijmegen breakage syndrome
Breast cancer
Aplastic anemia
Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 FHA domain. -
Domain
The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. -
Post-translational
modificationsPhosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. -
Cellular localization
Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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Database links
- Entrez Gene: 4683 Human
- Entrez Gene: 27354 Mouse
- Omim: 602667 Human
- SwissProt: O60934 Human
- SwissProt: Q9R207 Mouse
- Unigene: 492208 Human
- Unigene: 20866 Mouse
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Alternative names
- AT V1 antibody
- AT V2 antibody
- ATV antibody
see all
Images
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All lanes : Anti-p95/NBS1 antibody (ab175800)
Lane 1 : Control si
Lane 2 : Nbs1 si
Lane 3 : NIH 3T3 cell lysate at 40 µg -
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling p95/NBS1 with ab175800 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - p95/NBS1, Middle - DAPI, Right - Merge.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody (ab175800)
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling p95/NBS1 with ab175800 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Right - 20X.
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Immunoprecipitation analysis of U2OS cells labelling p95/NBS1 with ab175800. Protein A beads were crosslinked to the serum.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab175800 has been referenced in 3 publications.
- Alonso-de Vega I et al. PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaks. Nucleic Acids Res N/A:N/A (2020). WB ; Human . PubMed: 32232336
- Warmerdam DO et al. PHF6 promotes non-homologous end joining and G2 checkpoint recovery. EMBO Rep N/A:e48460 (2019). WB ; Human . PubMed: 31782600
- Altan B et al. High Expression of MRE11-RAD50-NBS1 Is Associated with Poor Prognosis and Chemoresistance in Gastric Cancer. Anticancer Res 36:5237-5247 (2016). IHC-P ; Human . PubMed: 27798884