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  1. Link

    p95nbs1-antibody-ab175800.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Non Homol. End Joining
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Anti-p95/NBS1 antibody (ab175800)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-p95/NBS1 antibody (ab175800)
  • Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody (ab175800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody (ab175800)
  • Immunoprecipitation - Anti-p95/NBS1 antibody (ab175800)

Key features and details

  • Rabbit polyclonal to p95/NBS1
  • Suitable for: IP, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-p95/NBS1 antibody
    See all p95/NBS1 primary antibodies
  • Description

    Rabbit polyclonal to p95/NBS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • General notes

     This product was previously labelled as p95 NBS1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Constituent: Water
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human p95/NBS1 protein (ab158956)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-P
1/1000.
Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-P
1/1000.

Target

  • Function

    Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificity

    Ubiquitous. Expressed at high levels in testis.
  • Involvement in disease

    Nijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 FHA domain.
  • Domain

    The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    modifications

    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localization

    Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Target information above from: UniProt accession O60934 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4683 Human
    • Entrez Gene: 27354 Mouse
    • Omim: 602667 Human
    • SwissProt: O60934 Human
    • SwissProt: Q9R207 Mouse
    • Unigene: 492208 Human
    • Unigene: 20866 Mouse
    • Alternative names

      • AT V1 antibody
      • AT V2 antibody
      • ATV antibody
      • Cell cycle regulatory protein p95 antibody
      • FLJ10155 antibody
      • MGC87362 antibody
      • Nbn antibody
      • NBN_HUMAN antibody
      • NBS 1 antibody
      • NBS antibody
      • NBS1 antibody
      • Nibrin antibody
      • Nijmegen breakage syndrome 1 (nibrin) antibody
      • Nijmegen breakage syndrome antibody
      • Nijmegen breakage syndrome protein 1 antibody
      • p95 antibody
      • p95 protein of the MRE11/RAD50 complex antibody
      see all

    Images

    • Western blot - Anti-p95/NBS1 antibody (ab175800)
      Western blot - Anti-p95/NBS1 antibody (ab175800)
      All lanes : Anti-p95/NBS1 antibody (ab175800)

      Lane 1 : Control si
      Lane 2 : Nbs1 si
      Lane 3 : NIH 3T3 cell lysate at 40 µg
    • Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody (ab175800)
      Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody (ab175800)

      Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling p95/NBS1 with ab175800 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - p95/NBS1, Middle - DAPI, Right - Merge.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody (ab175800)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody (ab175800)

      Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling p95/NBS1 with ab175800 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Right - 20X.

    • Immunoprecipitation - Anti-p95/NBS1 antibody (ab175800)
      Immunoprecipitation - Anti-p95/NBS1 antibody (ab175800)

      Immunoprecipitation analysis of U2OS cells labelling p95/NBS1 with ab175800. Protein A beads were crosslinked to the serum.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab175800? Please let us know so that we can cite the reference in this datasheet.

    ab175800 has been referenced in 3 publications.

    • Alonso-de Vega I  et al. PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaks. Nucleic Acids Res N/A:N/A (2020). WB ; Human . PubMed: 32232336
    • Warmerdam DO  et al. PHF6 promotes non-homologous end joining and G2 checkpoint recovery. EMBO Rep N/A:e48460 (2019). WB ; Human . PubMed: 31782600
    • Altan B  et al. High Expression of MRE11-RAD50-NBS1 Is Associated with Poor Prognosis and Chemoresistance in Gastric Cancer. Anticancer Res 36:5237-5247 (2016). IHC-P ; Human . PubMed: 27798884

    Customer reviews and Q&As

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