Overview

  • Product name

  • Description

    Rabbit polyclonal to p95/NBS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human p95/NBS1 (internal sequence). The exact sequence is proprietary.
    Database link: O60934

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2 and NTERA-2 cl.D1 [NT2/D1] whole cell extracts. IP: NTERA-2 cl.D1 [NT2/D1] whole cell extract. ICC/IF: A431 cells.
  • General notes

     This product was previously labelled as p95 NBS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 85 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificity

    Ubiquitous. Expressed at high levels in testis.
  • Involvement in disease

    Nijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 FHA domain.
  • Domain

    The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    modifications

    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localization

    Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT V1 antibody
    • AT V2 antibody
    • ATV antibody
    • Cell cycle regulatory protein p95 antibody
    • FLJ10155 antibody
    • MGC87362 antibody
    • Nbn antibody
    • NBN_HUMAN antibody
    • NBS 1 antibody
    • NBS antibody
    • NBS1 antibody
    • Nibrin antibody
    • Nijmegen breakage syndrome 1 (nibrin) antibody
    • Nijmegen breakage syndrome antibody
    • Nijmegen breakage syndrome protein 1 antibody
    • p95 antibody
    • p95 protein of the MRE11/RAD50 complex antibody
    see all

Images

  • All lanes : Anti-p95/NBS1 antibody (ab227068) at 1/2000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T, transfected with p95/NBS1 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • p95/NBS1 was immunoprecipitated from NTERA-2 cl.D1 [NT2/D1] (humanmalignant pluripotent embryonic carcinoma cell line) whole cell extract with 4 µg ab227068. Western blot was performed from the immunoprecipitate using ab227068 at 1/500 dilution.

    Lane 1: NTERA-2 cl.D1 [NT2/D1] whole cell extract 50 µg.

    Lane 2: Control IP in NTERA-2 cl.D1 [NT2/D1] whole cell extract with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227068 IP in NTERA-2 cl.D1 [NT2/D1] whole cell extract.

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for p95/NBS1 (green) using ab227068 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-p95/NBS1 antibody (ab227068) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Anti-p95/NBS1 antibody (ab227068) at 1/5000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 85 kDa



    7.5 % SDS-PAGE gel.

References

ab227068 has not yet been referenced specifically in any publications.

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