Key features and details
- Rabbit polyclonal to p95/NBS1 (phospho S278)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p95/NBS1 (phospho S278) antibody
See all p95/NBS1 primary antibodies
DescriptionRabbit polyclonal to p95/NBS1 (phospho S278)
ab111373 detects endogenous levels of p95/NBS1 only when phosphorylated at serine 278.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human p95/NBS1.
- HUVEC cell extracts, treated with foskolin; NIH3T3 cells.
This product was previously labelled as p95 NBS1
Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111373 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab111373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 84 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
Tissue specificityUbiquitous. Expressed at high levels in testis.
Involvement in diseaseNijmegen breakage syndrome
Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 BRCT domain.
Contains 1 FHA domain.
DomainThe FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
modificationsPhosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
Cellular localizationNucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
- Information by UniProt
- AT V1 antibody
- AT V2 antibody
- ATV antibody
All lanes : Anti-p95/NBS1 (phospho S278) antibody (ab111373) at 1/500 dilution
Lane 1 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins)
Lane 2 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
ab111373 at 1/100 dilution staining p95/NBS1 in NIH3T3 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
ab111373 has not yet been referenced specifically in any publications.