Anti-p95/NBS1 (phospho S343) antibody (ab47272)
Key features and details
- Rabbit polyclonal to p95/NBS1 (phospho S343)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-p95/NBS1 (phospho S343) antibody
See all p95/NBS1 primary antibodies -
Description
Rabbit polyclonal to p95/NBS1 (phospho S343) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human p95/NBS1 (phospho S343).
Database link: O60934 -
Positive control
- Jurkat cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 85 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 85 kDa). |
Target
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Function
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. -
Tissue specificity
Ubiquitous. Expressed at high levels in testis. -
Involvement in disease
Nijmegen breakage syndrome
Breast cancer
Aplastic anemia
Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 FHA domain. -
Domain
The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. -
Post-translational
modificationsPhosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. -
Cellular localization
Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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Database links
- Entrez Gene: 4683 Human
- Omim: 602667 Human
- SwissProt: O60934 Human
- Unigene: 492208 Human
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Alternative names
- AT V1 antibody
- AT V2 antibody
- ATV antibody
see all
Images
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All lanes : Anti-p95/NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution
Lane 1 : Unirradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 2 : Irradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 3 : Unirradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lane 4 : Irradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat polyclonal to rabbit IgG at 1/3000 dilution
Predicted band size: 85 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab47272 has been referenced in 11 publications.
- Blanchard-Rohner G et al. Childhood-Onset Movement Disorders Can Mask a Primary Immunodeficiency: 6 Cases of Classical Ataxia-Telangiectasia and Variant Forms. Front Immunol 13:791522 (2022). PubMed: 35154108
- Herok M et al. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance. Cancers (Basel) 13:N/A (2021). PubMed: 34572735
- Hollingworth R et al. Productive herpesvirus lytic replication in primary effusion lymphoma cells requires S-phase entry. J Gen Virol N/A:N/A (2020). PubMed: 32501196
- Weber AM et al. Phenotypic consequences of somatic mutations in the ataxia-telangiectasia mutated gene in non-small cell lung cancer. Oncotarget 7:60807-60822 (2016). WB . PubMed: 27602502
- Byrd PJ et al. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development. PLoS Genet 12:e1005945 (2016). WB . PubMed: 26990772