Product nameAnti-P97/DAP5 antibody
See all P97/DAP5 primary antibodies
DescriptionRabbit polyclonal to P97/DAP5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human P97/DAP5 aa 800-850.
Database link: NP_001409.1
- HeLa cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAppears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Tissue specificityUbiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Sequence similaritiesBelongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.
modificationsPhosphorylation; hyperphosphorylated during mitosis.
- Information by UniProt
- DAP-5 antibody
- DAP5 antibody
- Death associated protein 5 antibody
All lanes : Anti-P97/DAP5 antibody (ab85963) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 minutes
1mg HeLa whole cell lysate was immunoprecipitated using 10µg ab85963.
20% of IP was loaded per lane and probed with ab85963 at 0.4µg/ml (lane 1) and a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 30 seconds.