Key features and details
- Rabbit polyclonal to P97/DAP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-P97/DAP5 antibody
See all P97/DAP5 primary antibodies
DescriptionRabbit polyclonal to P97/DAP5
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
Recombinant fragment corresponding to Human P97/DAP5 aa 1-204.
Database link: P78344
- WB: A431 whole cell lysate. Mouse brain whole cell lysate. IHC-P: Cal27 xenograft, human skeletal muscle. Mouse hind brain tissue. IF/ICC: HeLa cell line.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 102 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionAppears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Tissue specificityUbiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Sequence similaritiesBelongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.
modificationsPhosphorylation; hyperphosphorylated during mitosis.
- Information by UniProt
- DAP-5 antibody
- DAP5 antibody
- Death associated protein 5 antibody
Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 102 kDa
5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution
HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.
Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.
Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa
7.5% SDS PAGE
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.
ab97302 has not yet been referenced specifically in any publications.