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Rabbit polyclonal to P97/DAP5
Predicted to work with:
Mouse, Rat, Rabbit, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 204 of Human DAP5 (NP_001409)
A431 whole cell lysate and Cal27.
IF/ICC: HeLa cell line.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 40% Glycerol, PBS, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 102 kDa.
Use a concentration of 10 µg/ml.
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Belongs to the eIF4G family.
Contains 1 MI domain. Contains 1 MIF4G domain. Contains 1 W2 domain.
Phosphorylation; hyperphosphorylated during mitosis.
Information by UniProt
Death associated protein 5 antibody
Immunocytochemistry/ Immunofluorescence - Anti-DAP5 antibody (ab97302)
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was
ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-DAP5 antibody (ab97302)
Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP5 antibody (ab97302)
Immunohistochemical analysis of paraffin-embedded Cal27, using ab97302 at 1/100 dilution
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"