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A user would like to use Anti-PABP antibody [10E10] (ab6125) on follicular fluids by ELISA. Could you please suggest a dilution rate for the antibody to use ELISA? Additionally, is PABP peptide (ab23710) suitable to use with PABP antibody [10E10] (ab6125) as standard?
Asked on Aug 29 2012
Thank you for contacting us.
There is not a specific concentration recommended for the use of this antibody in ELISA. Optimisation is required with different dilutions in order to assess the best one for your particular conditions. We can however suggest a good starting point to begin with, for purified antibodies tested in ELISA this corresponds to 0.1ug/ml.
Regarding the suitability of ab23710 to be used as standard with the antibody, I am afraid I cannot assure the antibody will recognize it, as its epitope has not been mapped, and we have not tested this pair in house.
However, the peptide ab23710 has been used to raise another Anti-PABP antibody:
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Aug 29 2012