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  1. Link

    pabp-antibody-ab21060.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-PABP antibody (ab21060)

  • Datasheet
  • SDS
Reviews (12)Q&A (1)References (104)

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Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
  • Western blot - Anti-PABP antibody (ab21060)
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
  • Western blot - Anti-PABP antibody (ab21060)
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

Key features and details

  • Rabbit polyclonal to PABP
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Human eIF4A1 peptide (ab31796)

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Overview

  • Product name

    Anti-PABP antibody
    See all PABP primary antibodies
  • Description

    Rabbit polyclonal to PABP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human PABP.

    Read Abcam's proprietary immunogen policy (Peptide available as ab23710.)
  • Positive control

    • ICC/IF: HeLa cells. WB: HeLa, NIH/3T3, MEF1 and PC12 whole cell lysate. Mouse brain, testis, spinal cord, ovary and pancreas tissue lysate. Brain (Rat) Tissue Lysate - normal tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • RNAi
    • P bodies/GW bodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa.
ICC/IF (4)
Use a concentration of 1 - 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa.
ICC/IF
Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). Positively regulates the replication of dengue virus (DENV) (PubMed:26735137).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the polyadenylate-binding protein type-1 family.
    Contains 1 PABC domain.
    Contains 4 RRM (RNA recognition motif) domains.
  • Domain

    The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively.
    The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively.
  • Post-translational
    modifications

    Phosphorylated by MAPKAPK2.
    Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm. Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the cytoplasm and the nucleus.
  • Target information above from: UniProt accession P11940 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 26986 Human
    • Entrez Gene: 18458 Mouse
    • Entrez Gene: 171350 Rat
    • Omim: 604679 Human
    • SwissProt: P11940 Human
    • SwissProt: P29341 Mouse
    • SwissProt: Q9EPH8 Rat
    • Unigene: 387804 Human
    • Unigene: 371570 Mouse
    • Unigene: 127813 Rat
    see all
  • Alternative names

    • PAB 1 antibody
    • PAB1 antibody
    • PABP 1 antibody
    • PABP-1 antibody
    • PABP1 antibody
    • PABP1_HUMAN antibody
    • PABPC 1 antibody
    • Pabpc1 antibody
    • PABPC2 antibody
    • PABPL1 antibody
    • Poly A binding protein 1 antibody
    • Poly A binding protein cytoplasmic 1 antibody
    • poly(A) binding protein, cytoplasmic 1 antibody
    • poly(A) binding protein, cytoplasmic 2 antibody
    • Poly(A)-binding protein 1 antibody
    • Polyadenylate binding protein 1 antibody
    • Polyadenylate-binding protein 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

    ab21060 staining PABP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21060 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-PABP antibody (ab21060)
    Western blot - Anti-PABP antibody (ab21060)
    Anti-PABP antibody (ab21060) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg/ml

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    ab21060 detects a band of ~ 75 kDa in HeLa whole cell lysate. A 75 kDa band is also seen in Jurkat and A431 whole cell lysates (data not shown). PABP is reported to have run at an apparent molecular mass of 73 kDa on SDS-PAGE. We believe the band we see at 75 kDa represents PABP.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)Sharma et al PLoS Pathog. 2017 Oct 30;13(10):e1006677. doi: 10.1371/journal.ppat.1006677. eCollection 2017 Oct. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Viral ORF57 expression is required to suppress the formation of SG during KSHV lytic infection and under arsenite stress.

    ORF57 alone is sufficient to inhibit SG formation. HeLa cells transfected with an ORF57-Flag (pVM7) expressing vector or an empty vector (control) for 24 h were induced with 0.5 mM arsenite for 30 min for SG formation. The cells were stained for ORF57 (green), SG-specific TIA-1 (red) and PABPC1 (white) (ab21060) by each corresponding antibody. The nuclei were counterstained with Hoechst stain. Scale bar = 10 μm.

  • Western blot - Anti-PABP antibody (ab21060)
    Western blot - Anti-PABP antibody (ab21060)
    All lanes : Anti-PABP antibody (ab21060) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 6 : Spinal Cord (Mouse) Tissue Lysate
    Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 8 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 9 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)This image is courtesy of an anonymous Abreview

    ab21060 at 1/50 staining HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF.

    HeLa cells fixed for 10 minutes with 1.9 % formaldehyde in 2 % sucrose in PBS then permeabilized for 10 minutes with 0.5% NP40 in PBS-10% sucrose. The cells were then blocked in horse serum and  incubated with the antibody for 1 hour. An Alexa Fluor ® 555 conjugated donkey anti-rabbit antibody was used as the secondary.

     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)This image is courtesy of an Abreview submitted by Sathya Thulasi Raman.

    Immunocytochemical analysis of newborn porcine tracheal epithelial cells, labeling PABP with ab21060.

    Cells were formaldehyde-fixed, permeabilized in cold methanol, and blocked with 5% horse serum in PBS for 40 minutes at 23°C. Immunostaining with primary anti-PABP diluted 1/250 for 12 hours at 4°C. 

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)This image is courtesy of an anonymous Abreview.

    Immunocytochemical analysis of rat OLN93 oligodendroglia cells, labeling PABP with ab21060.

    Cells were paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100, and blocked with 10% normal goat serum for 1 hour at 20°C. Immunostaining with anti-PABP diluted 1/200 overnight at 4°C.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (104)

Publishing research using ab21060? Please let us know so that we can cite the reference in this datasheet.

ab21060 has been referenced in 104 publications.

  • Jia JJ  et al. mTORC1 promotes TOP mRNA translation through site-specific phosphorylation of LARP1. Nucleic Acids Res 49:3461-3489 (2021). PubMed: 33398329
  • Shalini V  et al. Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids. Epigenetics Chromatin 14:3 (2021). PubMed: 33407810
  • Clancy A  et al. The deubiquitylase USP9X controls ribosomal stalling. J Cell Biol 220:N/A (2021). PubMed: 33507233
  • Gao B  et al. Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication. PLoS Pathog 17:e1008690 (2021). PubMed: 33635931
  • Munakata F  et al. Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex. Genes (Basel) 12:N/A (2021). PubMed: 33668648
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

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1-10 of 12 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Newborn Porcine Tracheal Epithelial cell line)
Permeabilization
Yes - Cold methanol
Specification
Newborn Porcine Tracheal Epithelial cell line
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sathya Thulasi Raman

Verified customer

Submitted Jan 11 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Permeabilization
No
Specification
Fibroblast
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 22 2015

Western blot abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (cg4 cell line)
Specification
cg4 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 03 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Rat Cell (OLN93 oligodendroglia cell line)
Specification
OLN93 oligodendroglia cell line
Permeabilization
Yes - 0,1% Triton X100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 06 2013

Flow Cytometry abreview for Anti-PABP antibody

Good
Abreviews
Abreviews
Application
Flow Cytometry
Sample
Human Cell (HeLa)
Specification
HeLa
Preparation
Cell harvesting/tissue preparation method: grown in TC
Sample buffer: harvested by scraping in cold PBS
Fixation
BD CytoFix/CytoPerm
Permeabilization
Yes - BD CytoFix/CytoPerm
Gating Strategy
single cells
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Lee Gehrke

Verified customer

Submitted Apr 10 2013

CLIP abreview for Anti-PABP antibody

Inconclusive
Abreviews
Abreviews
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 21 2012

Western blot abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLA)
Loading amount
60 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 27 2011

Western blot abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount
25 µg
Specification
Mouse Embryonic Fibroblasts
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 27 2011

Western blot abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse adipocyte)
Loading amount
5 µg
Specification
mouse adipocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 17 2011

Immunoprecipitation abreview for Anti-PABP antibody

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293)
Total protein in input
1000 µg
Specification
HEK293
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 12 2010

1-10 of 12 Abreviews

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