Overview

  • Product name

    Anti-PABPC3 antibody [EPR8998]
    See all PABPC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8998] to PABPC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PABPC3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, mouse testis, and rat testis lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8998
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1.
    • Tissue specificity

      Testis specific.
    • Sequence similarities

      Belongs to the polyadenylate-binding protein type-1 family.
      Contains 1 PABC domain.
      Contains 4 RRM (RNA recognition motif) domains.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • PABP-3 antibody
      • PABP3 antibody
      • PABP3_HUMAN antibody
      • PABPC3 antibody
      • PABPL3 antibody
      • Poly(A) binding protein 3 antibody
      • Poly(A)-binding protein 3 antibody
      • Polyadenylate binding protein 3 antibody
      • Polyadenylate-binding protein 3 antibody
      • RP11-165I9.5 antibody
      • Testis specific poly(A) binding protein antibody
      • Testis-specific poly(A)-binding protein antibody
      • tPABP antibody
      see all

    Images

    • All lanes : Anti-PABPC3 antibody [EPR8998] (ab134967) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Rat testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 70 kDa

    • Immunofluorescent analysis of Jurkat cells labelling PABPC3 with ab134967 at 1/50 dilution .

    References

    ab134967 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134967.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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